Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Soares, Júlia Ribeiro | - |
| dc.creator | José Tenório de Melo, Edésio | - |
| dc.creator | da Cunha, Maura | - |
| dc.creator | Fernandes, Kátia Valevski Sales | - |
| dc.creator | Taveira, Gabriel Bonan | - |
| dc.creator | da Silva Pereira, Lidia | - |
| dc.creator | Pimenta, Samy | - |
| dc.creator | Trindade, Fernanda Gomes | - |
| dc.creator | Regente, Mariana Clelia | - |
| dc.creator | Pinedo, Marcela Lilian | - |
| dc.creator | de la Canal, Laura | - |
| dc.creator | Gomes, Valdirene Moreira | - |
| dc.creator | de Oliveira Carvalho, André | - |
| dc.date | 2018-11-28T18:06:24Z | - |
| dc.date | 2018-11-28T18:06:24Z | - |
| dc.date | 2017-01 | - |
| dc.date | 2018-10-23T14:12:28Z | - |
| dc.date.accessioned | 2019-04-29T15:46:35Z | - |
| dc.date.available | 2019-04-29T15:46:35Z | - |
| dc.date.issued | 2017-01 | - |
| dc.identifier | Soares, Júlia Ribeiro; José Tenório de Melo, Edésio; da Cunha, Maura; Fernandes, Kátia Valevski Sales; Taveira, Gabriel Bonan; et al.; Interaction between the plant ApDef1 defensin and Saccharomyces cerevisiae results in yeast death through a cell cycle- and caspase-dependent process occurring via uncontrolled oxidative stress; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1861; 1; 1-2017; 3429-3443 | - |
| dc.identifier | 0304-4165 | - |
| dc.identifier | http://hdl.handle.net/11336/65475 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/301698 | - |
| dc.description | Background Plant defensins were discovered at beginning of the 90s' however, their precise mechanism of action is still unknown. Herein, we studied ApDef1-Saccharomyces cerevisiae interaction. Methods ApDef1-S. cerevisiae interaction was studied by determining the MIC, viability and death kinetic assays. Viability assay was repeated with hydroxyurea synchronized-yeast and pretreated with CCCP. Plasma membrane permeabilization, ROS induction, chromatin condensation, and caspase activation analyses were assessed through Sytox green, DAB, DAPI and FITC-VAD-FMK, respectively. Viability assay was done in presence of ascorbic acid and Z-VAD-FMK. Ultrastructural analysis was done by electron microscopy. Results ApDef1 caused S. cerevisiae cell death and MIC was 7.8 μM. Whole cell population died after 18 h of ApDef1 interaction. After 3 h, 98.76% of synchronized cell population died. Pretreatment with CCCP protected yeast from ApDef1 induced death. ApDef1-S. cerevisiae interaction resulted in membrane permeabilization, H2O2 increased production, chromatin condensation and caspase activation. Ascorbic acid prevented yeast cell death and membrane permeabilization. Z-VAD-FMK prevented yeast cell death. Conclusions ApDef1-S. cerevisiae interaction caused cell death through cell cycle dependentprocess which requires preserved membrane potential. After interaction, yeast went through uncontrolled ROS production and accumulation, which led to plasma membrane permeabilization, chromatin condensation and, ultimately, cell death by activation of caspase-dependent apoptosis via. General significance We show novel requirements for the interaction between plant defensin and fungi cells, i.e. cell cycle phase and membrane potential, and we indicate that membrane permeabilization is probably caused by ROS and therefore, it would be an indirect event of the ApDef1-S. cerevisiae interaction. | - |
| dc.description | Fil: Soares, Júlia Ribeiro. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: José Tenório de Melo, Edésio. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: da Cunha, Maura. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: Fernandes, Kátia Valevski Sales. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: Taveira, Gabriel Bonan. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: da Silva Pereira, Lidia. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: Pimenta, Samy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: Trindade, Fernanda Gomes. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina | - |
| dc.description | Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina | - |
| dc.description | Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina | - |
| dc.description | Fil: Gomes, Valdirene Moreira. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.description | Fil: de Oliveira Carvalho, André. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbagen.2016.09.005 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304416516303270 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | ANTIMICROBIAL PEPTIDES | - |
| dc.subject | CASPASE | - |
| dc.subject | CELL CYCLE | - |
| dc.subject | MEMBRANE PERMEABILIZATION | - |
| dc.subject | MICROBICIDAL | - |
| dc.subject | ROS INDUCTION | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Interaction between the plant ApDef1 defensin and Saccharomyces cerevisiae results in yeast death through a cell cycle- and caspase-dependent process occurring via uncontrolled oxidative stress | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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