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dc.creatorBreccia, Gabriela-
dc.creatorVega, Tatiana Alejandra-
dc.creatorFelitti, Silvina Andrea-
dc.creatorPicardi, Liliana Amelia-
dc.creatorNestares, Graciela María-
dc.date2017-07-21T20:23:13Z-
dc.date2017-07-21T20:23:13Z-
dc.date2013-03-
dc.date2017-07-21T19:05:22Z-
dc.date.accessioned2019-04-29T15:46:38Z-
dc.date.available2019-04-29T15:46:38Z-
dc.date.issued2013-03-
dc.identifierBreccia, Gabriela; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Picardi, Liliana Amelia; Nestares, Graciela María; Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide; Elsevier Ireland; Plant Science; 208; 3-2013; 28-33-
dc.identifier0168-9452-
dc.identifierhttp://hdl.handle.net/11336/21133-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/301729-
dc.descriptionAcetohydroxyacid synthase (AHAS) catalyzes the first reaction in branch chain amino acids biosynthesis. This enzyme is the target of several herbicides, including all members of the imidazolinone family. Little is known about the expression of the three acetohydroxyacid synthase genes (ahas1, ahas2 and ahas3) in sunflower. The aim of this work was to evaluate ahas gene expression and AHAS activity in different tissues of sunflower plantlets. Three genotypes differing in imidazolinone resistance were evaluated, two of which carry an herbicide resistant-endowing mutation known as Ahasl1-1 allele. In vivo and in vitro AHAS activity and transcript levels were higher in leaves than in roots. The ahas3 transcript was the less abundant in both tissues. No significant difference was observed between ahas1 and ahas2 transcript levels of the susceptible genotype but a higher ahas1 transcript level was observed in leaves of genotypes carrying Ahasl1-1 allele. Similar transcript levels were found for ahas1 and ahas2 in roots of genotypes carrying Ahasl1-1 allele whereas higher ahas2 abundance was found in the susceptible genotype. Herbicide treatment triggered tissue-specific, gene and genotype-dependent changes in ahas gene expression. AHAS activity was highly inhibited in the susceptible genotype. Differential responses were observed between in vitro and in vivo AHAS inhibition assays. These findings enhance our understanding of AHAS expression in sunflower genotypes differing for herbicide resistance and its response to herbicide treatment.-
dc.descriptionFil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Vega, Tatiana Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Picardi, Liliana Amelia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina-
dc.descriptionFil: Nestares, Graciela María. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
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dc.languageeng-
dc.publisherElsevier Ireland-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plantsci.2013.03.008-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945213000642-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectHelianthus annuus L.-
dc.subjectImidazolinone herbicide-
dc.subjectahas gene expression-
dc.subjectAHAS activity-
dc.subjectHerbicide tolerance-
dc.subjectAgronomía, reproducción y protección de plantas-
dc.subjectAgricultura, Silvicultura y Pesca-
dc.subjectCIENCIAS AGRÍCOLAS-
dc.subjectBiotecnología Agrícola y Biotecnología Alimentaria-
dc.subjectBiotecnología Agropecuaria-
dc.subjectCIENCIAS AGRÍCOLAS-
dc.titleDifferential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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