Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Lardone, Ricardo Dante | - |
| dc.creator | Chan, Alfred A. | - |
| dc.creator | Lee, Agnes F. | - |
| dc.creator | Foshag, Leland J. | - |
| dc.creator | Faries, Mark B. | - |
| dc.creator | Sieling, Peter A. | - |
| dc.creator | Lee, Delphine J. | - |
| dc.date | 2018-08-29T17:11:41Z | - |
| dc.date | 2018-08-29T17:11:41Z | - |
| dc.date | 2017-08 | - |
| dc.date | 2018-08-27T18:57:32Z | - |
| dc.date.accessioned | 2019-04-29T15:46:45Z | - |
| dc.date.available | 2019-04-29T15:46:45Z | - |
| dc.date.issued | 2017-08 | - |
| dc.identifier | Lardone, Ricardo Dante; Chan, Alfred A.; Lee, Agnes F.; Foshag, Leland J.; Faries, Mark B.; et al.; Mycobacterium bovis Bacillus Calmette–Guérin Alters melanoma microenvironment Favoring antitumor T cell responses and improving M2 Macrophage Function; Frontiers Media S.A.; Frontiers in Immunology; 8; 8-2017 | - |
| dc.identifier | http://hdl.handle.net/11336/57514 | - |
| dc.identifier | 1664-3224 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/301779 | - |
| dc.description | Intralesional Mycobacterium bovis bacillus Calmette–Guérin (BCG) has long been a relatively inexpensive therapy for inoperable cutaneous metastatic melanoma (CMM), although intralesional BCG skin mechanisms remain understudied. We analyzed intralesional BCG-treated CMM lesions combined with in vitro studies to further investigate BCG-altered pathways. Since macrophages play a pivotal role against both cancer and mycobacterial infections, we hypothesized BCG regulates macrophages to promote antitumor immunity. Tumor-associated macrophages (M2) infiltrate melanomas and impair antitumor immunity. BCG-treated, in vitro-polarized M2 (M2-BCG) showed transcriptional changes involving inflammation, immune cell recruitment, cross talk, and activation pathways. Mechanistic network analysis indicated M2-BCG potential to improve interferon gamma (IFN-γ) responses. Accordingly, frequency of IFN-γ-producing CD4+ T cells responding to M2-BCG vs. mock-treated M2 increased (p < 0.05). Moreover, conditioned media from M2-BCG vs. M2 elevated the frequency of granzyme B-producing CD8+ tumor-infiltrating lymphocytes (TILs) facing autologous melanoma cell lines (p < 0.01). Furthermore, transcriptome analysis of intralesional BCG-injected CMM relative to uninjected lesions showed immune function prevalence, with the most enriched pathways representing T cell activation mechanisms. In vitro-infected MM-derived cell lines stimulated higher frequency of IFN-γ-producing TIL from the same melanoma (p < 0.05). Our data suggest BCG favors antitumor responses in CMM through direct/indirect effects on tumor microenvironment cell types including macrophages, T cells, and tumor itself. | - |
| dc.description | Fil: Lardone, Ricardo Dante. The John Wayne Cancer Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina | - |
| dc.description | Fil: Chan, Alfred A.. The John Wayne Cancer Institute; Estados Unidos. Los Angeles Biomedical Research Institute At Harborucla; Estados Unidos | - |
| dc.description | Fil: Lee, Agnes F.. The John Wayne Cancer Institute; Estados Unidos | - |
| dc.description | Fil: Foshag, Leland J.. The John Wayne Cancer Institute; Estados Unidos | - |
| dc.description | Fil: Faries, Mark B.. The John Wayne Cancer Institute; Estados Unidos | - |
| dc.description | Fil: Sieling, Peter A.. The John Wayne Cancer Institute; Estados Unidos | - |
| dc.description | Fil: Lee, Delphine J.. Los Angeles Biomedical Research Institute At Harborucla; Estados Unidos. The John Wayne Cancer Institute; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Frontiers Media S.A. | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fimmu.2017.00965/abstract | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fimmu.2017.00965 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | ANTITUMOR IMMUNITY MECHANISMS | - |
| dc.subject | CUTANEOUS METASTATIC MELANOMA | - |
| dc.subject | INTRALESIONAL BACILLUS CALMETTE–GUÉRIN | - |
| dc.subject | MELANOMA MICROENVIRONMENT | - |
| dc.subject | T CELL RESPONSE | - |
| dc.subject | Inmunología | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Mycobacterium bovis Bacillus Calmette–Guérin Alters melanoma microenvironment Favoring antitumor T cell responses and improving M2 Macrophage Function | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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