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dc.provenanceCONICET-
dc.creatorPellegrini Malpiedi, Luciana-
dc.creatorNerli, Bibiana Beatriz-
dc.creatorAbdalla, Dulcinéia Saes Parra-
dc.creatorPessoa, Adalberto Jr.-
dc.date2017-12-12T17:18:39Z-
dc.date2017-12-12T17:18:39Z-
dc.date2014-05-
dc.date2017-12-07T15:42:26Z-
dc.date.accessioned2019-04-29T15:47:36Z-
dc.date.available2019-04-29T15:47:36Z-
dc.date.issued2014-05-
dc.identifierPellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-561-
dc.identifier8756-7938-
dc.identifierhttp://hdl.handle.net/11336/30302-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302154-
dc.descriptionThe effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures-
dc.descriptionFil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina-
dc.descriptionFil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina-
dc.descriptionFil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil-
dc.descriptionFil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; Brasil-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAmerican Chemical Society-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/btpr.1882-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1882/abstract-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/30302-
dc.subjectNONIONIC SURFACTANT-
dc.subjectSINGLE-CHAIN ANTIBODY FRAGMENT-
dc.subjectSPECTROSCOPIC STUDY-
dc.subjectOtras Ciencias Químicas-
dc.subjectCiencias Químicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleAssessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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