Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Yeves, Alejandra del Milagro | - |
| dc.creator | Villa-Abrille, María Celeste | - |
| dc.creator | Perez, Nestor Gustavo | - |
| dc.creator | Medina, Andrés Javier | - |
| dc.creator | Escudero, Eduardo M. | - |
| dc.creator | Ennis, Irene Lucia | - |
| dc.date | 2018-01-19T19:10:29Z | - |
| dc.date | 2018-01-19T19:10:29Z | - |
| dc.date | 2014-09 | - |
| dc.date | 2018-01-18T17:21:01Z | - |
| dc.date.accessioned | 2019-04-29T15:48:21Z | - |
| dc.date.available | 2019-04-29T15:48:21Z | - |
| dc.date.issued | 2014-09 | - |
| dc.identifier | Yeves, Alejandra del Milagro; Villa-Abrille, María Celeste; Perez, Nestor Gustavo; Medina, Andrés Javier; Escudero, Eduardo M.; et al.; Physiological cardiac hypertrophy: Critical role of AKT in the prevention of NHE-1 hyperactivity; Elsevier; Journal of Molecular and Cellular Cardiology; 76; 9-2014; 186-195 | - |
| dc.identifier | 0022-2828 | - |
| dc.identifier | http://hdl.handle.net/11336/34005 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302516 | - |
| dc.description | Background: The involvement of NHE-1 hyperactivity, critical for pathological cardiac hypertrophy (CH), in physiological CH has not been elucidated yet. Stimulation of NHE-1 increases intracellular Na+ and Ca2 + favouring calcineurin activation. Since myocardial stretch, an activator of NHE-1, is common to both types of CH, we speculate that NHE-1 hyperactivity may also happen in physiological CH. However, calcineurin activation is characteristic only for pathological hypertrophy. We hypothesize that an inhibitory AKT-dependent mechanism prevents NHE-1 hyperactivity in the setup of physiological CH. Methods: Physiological CH was induced in rats by swimming (90 min/day, 12 weeks) or in cultured isolated cardiomyocytes with IGF-1 (10 nmol/L). Results: Training induced eccentric CH development (left ventricular weight/tibial length: 22.0 ± 0.3 vs. 24.3 ± 0.7 mg/mm; myocyte cross sectional area: 100 ± 3.2 vs. 117 ± 4.1 %; sedentary (Sed) and swim-trained (Swim) respectively; p < 0.05] with decreased myocardial stiffness and collagen deposition [1.7 ± 0.05 % (Sed) vs. 1.4 ± 0.09 % (Swim); p < 0.05]. Increased phosphorylation of AKT, ERK1/2, p90RSK and NHE-1 at the consensus site for ERK1/2-p90RSK were detected in the hypertrophied hearts (P-AKT: 134 ± 10 vs. 100 ± 5; P-ERK1/2: 164 ± 17 vs. 100 ± 18; P-p90RSK: 160 ± 18 vs. 100 ± 9; P-NHE-1 134 ± 10 vs. 100 ± 10; % in Swim vs. Sed respectively; p < 0.05). No significant changes were detected neither in calcineurin activation [calcineurin Aβ 100 ± 10 (Sed) vs. 96 ± 12 (Swim)], nor NFAT nuclear translocation [100 ± 3.11 (Sed) vs. 95 ± 9.81 % (Swim)] nor NHE-1 expression [100 ± 8.5 (Sed) vs. 95 ± 6.7 % (Swim)]. Interestingly, the inhibitory phosphorylation of the NHE-1 consensus site for AKT was increased in the hypertrophied myocardium (151.6 ± 19.4 (Swim) vs. 100 ± 9.5 % (Sed); p < 0.05). In isolated cardiomyocytes 24 hours IGF-1 increased cell area (114 ± 1.3 %; p < 0.05) and protein/DNA content (115 ± 3.9 %, p < 0.05), effects not abolished by NHE-1 inhibition with cariporide (114 ± 3 and 117 ± 4.4 %, respectively). IGF-1 significantly decreased NHE-1 activity during pHi recovery from sustained intracellular acidosis (JH + at pHi 6.8: 4.08 ± 0.74 and 9.09 ± 1.21 mmol/L/min, IGF-1 vs. control; p < 0.05), and abolished myocardial slow force response, the mechanical counterpart of stretch-induced NHE-1 activation. Conclusions: NHE-1 hyperactivity seems not to be involved in physiological CH development, contrary to what characterizes pathological CH. We propose that AKT, through an inhibitory phosphorylation of the NHE-1, prevents its stretch-induced activation. This posttranslational modification emerges as an adaptive mechanism that avoids NHE-1 hyperactivity preserving its housekeeping functioning. | - |
| dc.description | Fil: Yeves, Alejandra del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.description | Fil: Villa-Abrille, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.description | Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.description | Fil: Medina, Andrés Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.description | Fil: Escudero, Eduardo M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.description | Fil: Ennis, Irene Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares ; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.yjmcc.2014.09.004 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022282814002855 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/20004 | - |
| dc.subject | NHE-1 | - |
| dc.subject | Physiological cardiac hypertrophy | - |
| dc.subject | AKT | - |
| dc.subject | IGF-1 | - |
| dc.subject | Exercise training | - |
| dc.subject | Inmunología | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Physiological cardiac hypertrophy: Critical role of AKT in the prevention of NHE-1 hyperactivity | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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