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dc.creatorAcosta, Eliana Gisela-
dc.creatorCastilla, Viviana-
dc.creatorDamonte, Elsa Beatriz-
dc.date2018-12-13T19:03:44Z-
dc.date2018-12-13T19:03:44Z-
dc.date2011-09-
dc.date2018-11-05T19:18:23Z-
dc.date.accessioned2019-04-29T15:48:54Z-
dc.date.available2019-04-29T15:48:54Z-
dc.date.issued2011-09-
dc.identifierAcosta, Eliana Gisela; Castilla, Viviana; Damonte, Elsa Beatriz; Infectious dengue-1 virus entry into mosquito C6/36 cells; Elsevier Science; Virus Research; 160; 1-2; 9-2011; 173-179-
dc.identifier0168-1702-
dc.identifierhttp://hdl.handle.net/11336/66445-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302768-
dc.descriptionThe entry of dengue virus-1 (DENV-1) strain Hawaii into mosquito C6/36 cells was analyzed using a variety of biochemical inhibitors together with electron microscopy. The treatment with ammonium chloride, chlorpromazine, dansylcadaverine and dynasore inhibited virus yields, determined by infectivity titrations, whereas nystatin and methyl-β-cyclodextrin did not have any effect. The effect of the clathrin and dynamin inhibitors on DENV-1 entry was corroborated by detection of internalized virions using immunofluorescence staining. Furthermore, electron micrographs showed the incoming virions attached to electron-dense invaginations of the plasma membrane and within coated vesicles that resembled clathrin-coated pits and vesicles, respectively. The susceptibility to clathrin and dynamin inhibitors of clinical isolates from recent outbreaks was comparable to that shown by the cell culture-adapted reference strain. Similarly, DENV-3 strain H87 and DENV-4 strain 8124 were also inhibited in the presence of ammonium chloride, chlorpromazine and dynasore, allowing conclude that the infectious entry of DENV serotypes to mosquito cells occurs by low pH-dependent clathrin-mediated endocytosis. © 2011 Elsevier B.V.-
dc.descriptionFil: Acosta, Eliana Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina-
dc.descriptionFil: Castilla, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina-
dc.descriptionFil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170211002310-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.virusres.2011.06.008-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectCLATHRIN-MEDIATED ENDOCYTOSIS-
dc.subjectDENGUE VIRUS-1-
dc.subjectMOSQUITO CELLS-
dc.subjectVIRUS ENTRY-
dc.subjectSalud Ocupacional-
dc.subjectCiencias de la Salud-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleInfectious dengue-1 virus entry into mosquito C6/36 cells-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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