Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Parisi, Monica | - |
| dc.creator | Moreno, Silvia | - |
| dc.creator | Fernández, Graciela | - |
| dc.date | 2018-06-12T18:22:43Z | - |
| dc.date | 2018-06-12T18:22:43Z | - |
| dc.date | 2002-11 | - |
| dc.date | 2018-05-10T15:46:11Z | - |
| dc.date.accessioned | 2019-04-29T15:49:14Z | - |
| dc.date.available | 2019-04-29T15:49:14Z | - |
| dc.date.issued | 2002-11 | - |
| dc.identifier | Parisi, Monica; Moreno, Silvia; Fernández, Graciela; Characterization of a novel peptidase from tissue culture of garlic (Allium sativum.L); Springer; In Vitro Cellular ; 38; 6; 11-2002; 608-612 | - |
| dc.identifier | 1054-5476 | - |
| dc.identifier | http://hdl.handle.net/11336/48383 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302909 | - |
| dc.description | A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease expression. The protease activity belongs to the class of cysteine proteases since they are inhibited by E64 and Leupeptin and also they are activated by 2-mercaptoethanol and cysteine. They showed good thermal stability. Three active protease bands were found in zymograms of Allium sativum. The in vitro system revealed a significantly higher protease level than storage and embryo tissues of in vivo bulbs. | - |
| dc.description | Fil: Parisi, Monica. Universidad Nacional de Luján; Argentina | - |
| dc.description | Fil: Moreno, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina | - |
| dc.description | Fil: Fernández, Graciela. Universidad Nacional de Luján; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Springer | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1079/IVP2002344 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1079/IVP2002344 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | CALLUS CULTURE | - |
| dc.subject | CYSTEINE PROTEASE | - |
| dc.subject | ENDOPEPTIDASE | - |
| dc.subject | IN VITRO CULTURE | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Characterization of a novel peptidase from tissue culture of garlic (Allium sativum.L) | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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