Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Cappa, Valeria Alejandra | - |
| dc.creator | Rivero, Cintia Wanda | - |
| dc.creator | Britos, Claudia Noelia | - |
| dc.creator | Martínez, Luis M. | - |
| dc.creator | Lozano, Mario Enrique | - |
| dc.creator | Trelles, Jorge Abel | - |
| dc.date | 2018-01-11T19:51:15Z | - |
| dc.date | 2018-01-11T19:51:15Z | - |
| dc.date | 2014-04 | - |
| dc.date | 2018-01-11T13:48:27Z | - |
| dc.date.accessioned | 2019-04-29T15:49:15Z | - |
| dc.date.available | 2019-04-29T15:49:15Z | - |
| dc.date.issued | 2014-04 | - |
| dc.identifier | Cappa, Valeria Alejandra; Trelles, Jorge Abel; Rivero, Cintia Wanda; Lozano, Mario Enrique; Martínez, Luis M.; Britos, Claudia Noelia; et al.; An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate; Elsevier; Process Biochemistry; 49; 7; 4-2014; 1169-1175 | - |
| dc.identifier | 1359-5113 | - |
| dc.identifier | http://hdl.handle.net/11336/33028 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302919 | - |
| dc.description | An efficient and green immobilized biocatalyst is herein reported to obtain 5-fluorouracil-2′-deoxyriboside (5FUradRib), an antimetabolite known as Floxuridine, used in gastrointestinal cancer treatment. Alginate is a natural polysaccharide used in the pharmaceutical industry due to its physicochemical properties, biocompatibility and non-toxicity. Multivalent cations, exposure time and cross-linking solution concentration were optimized, being Sr2+, 2 h and 0.2 M the best immobilization conditions. Furthermore, compression strength, swelling ratio and fracture frequency were evaluated, improving the mechanical stability of the biocatalyst favoring a future scale-up. On the other hand, the reaction parameters for 5FUradRib biosynthesis were optimized in order to obtain an immobilized biocatalyst with enhanced activity. Thus, Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate showed yields of 96% at short reaction times. The obtained biocatalyst was stable for more than 25 days in storage conditions (4 °C) and could be reused at least 10 times without loss of its activity. Additionally, Sr-alginate biocatalyst stability was evaluated in different organic solvents to obtain hydrophobic compounds such as 5-bromouracil-2′-deoxyriboside (5BrUradRib), an effective radiosensitizing agent used in anti-cancer therapy, being hexane the best co-solvent. Finally, a smooth, cheap and environmentally friendly method to obtain anti-cancer drugs was developed in this study. | - |
| dc.description | Fil: Cappa, Valeria Alejandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina | - |
| dc.description | Fil: Britos, Claudia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina | - |
| dc.description | Fil: Martínez, Luis M.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina | - |
| dc.description | Fil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina | - |
| dc.description | Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioprocesos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2014.03.029 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511314002244 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Lactic-acid-bacteria | - |
| dc.subject | Halogenated-2′-deoxyribosides | - |
| dc.subject | Microorganism-stabilization | - |
| dc.subject | Anti-tumoral-compounds | - |
| dc.subject | Co-solvents | - |
| dc.subject | Otras Biotecnologías de la Salud | - |
| dc.subject | Biotecnología de la Salud | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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