Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Wertheimer Hermitte, Eva Victoria | - |
| dc.creator | Krapf, Dario | - |
| dc.creator | de la Vega Beltran, José L. | - |
| dc.creator | Sánchez Cárdenas, Claudia | - |
| dc.creator | Navarrete, Felipe | - |
| dc.creator | Haddad, Douglas | - |
| dc.creator | Escoffier, Jessica | - |
| dc.creator | Salicioni, Ana M. | - |
| dc.creator | Levin, Lonny R. | - |
| dc.creator | Buck, Jochen | - |
| dc.creator | Mager, Jesse | - |
| dc.creator | Darszon, Alberto | - |
| dc.creator | Visconti, Pablo E. | - |
| dc.date | 2017-03-14T14:25:03Z | - |
| dc.date | 2017-03-14T14:25:03Z | - |
| dc.date | 2013-12 | - |
| dc.date | 2017-03-06T15:25:16Z | - |
| dc.date.accessioned | 2019-04-29T15:49:45Z | - |
| dc.date.available | 2019-04-29T15:49:45Z | - |
| dc.date.issued | 2013-12 | - |
| dc.identifier | Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; et al.; Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 49; 12-2013; 35307-35320 | - |
| dc.identifier | http://hdl.handle.net/11336/13822 | - |
| dc.identifier | 1083-351X | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303141 | - |
| dc.description | Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum. | - |
| dc.description | Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina | - |
| dc.description | Fil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; México | - |
| dc.description | Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México | - |
| dc.description | Fil: Navarrete, Felipe. University Of Massachussets; Estados Unidos | - |
| dc.description | Fil: Haddad, Douglas. University Of Massachussets; Estados Unidos | - |
| dc.description | Fil: Escoffier, Jessica. University Of Massachussets; Estados Unidos | - |
| dc.description | Fil: Salicioni, Ana M.. University Of Massachussets; Estados Unidos | - |
| dc.description | Fil: Levin, Lonny R.. Cornell University; Estados Unidos | - |
| dc.description | Fil: Buck, Jochen. Cornell University; Estados Unidos | - |
| dc.description | Fil: Mager, Jesse. University Of Massachussets; Estados Unidos | - |
| dc.description | Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México | - |
| dc.description | Fil: Visconti, Pablo E.. University Of Massachussets; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Biochemistry and Molecular Biology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/early/2013/10/15/jbc.M113.489476 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M113.489476 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | acrosome reaction | - |
| dc.subject | adenilate cyclase (adenylyl cyclase) | - |
| dc.subject | calcium imaging | - |
| dc.subject | cell signaling | - |
| dc.subject | cyclic AMP (cAMP) | - |
| dc.subject | forskolin | - |
| dc.subject | heterotrimeric G proteins | - |
| dc.subject | protein kinase A (PKA) | - |
| dc.subject | signal transduction | - |
| dc.subject | sperm capacitation | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Compartmentalization of distinct cAMP signaling pathways in mammalian sperm. | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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