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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Las Peñas, Maria Laura | - |
| dc.creator | Urdampilleta, Juan Domingo | - |
| dc.creator | López- Carro, B. | - |
| dc.creator | Santiñaque, F. | - |
| dc.creator | Kiesling, Roberto | - |
| dc.creator | Bernardello, Gabriel Luis Mario | - |
| dc.date | 2015-07-23T13:03:17Z | - |
| dc.date | 2015-07-23T13:03:17Z | - |
| dc.date | 2013-08 | - |
| dc.date | 2016-03-30 10:35:44.97925-03 | - |
| dc.date.accessioned | 2019-04-29T15:50:16Z | - |
| dc.date.available | 2019-04-29T15:50:16Z | - |
| dc.date.issued | 2013-08 | - |
| dc.identifier | Las Peñas, Maria Laura; Urdampilleta, Juan Domingo; López- Carro, B.; Santiñaque, F.; Kiesling, Roberto; et al.; Classical and molecular cytogenetics and DNA content in Maihuenia and Pereskia (Cactaceae); Springer Wien; Plant Systematics And Evolution; 8-2013; 1-10 | - |
| dc.identifier | 0378-2697 | - |
| dc.identifier | http://hdl.handle.net/11336/1422 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303350 | - |
| dc.description | We studied cacti species of the subfamilies Pereskioideae (five species of the southern clade) and both species of Maihuenioideae using molecular cytogenetic techniques and DNA content. Mitotic chromosomes were analyzed for Pereskia aculeata, P. bahiensis, P. grandifolia, P. nemorosa, P. sacharosa, Maihuenia poeppigii, and M. patagonica, using the Feulgen stain, CMA/DAPI fluorescent chromosome banding, fluorescence in situ hybridization (FISH, probes of 5S rDNA and pTa71 for 18-5.8-26S rDNA), and DNA content by flow cytometry technique. The karyotypes were highly symmetrical, most of the pairs being metacentric (m). CMA/DAPI banding revealed the presence of CMA+/DAPI- bands associated with NORs in the first m pair of all species. The colocalization of 18-5.8-26S rDNA loci with CMA+/DAPI-/ NORs blocks allowed the identification of homeologous chromosome pairs between species of both subfamilies. FISH using probe 5S rDNA was applied for the first time in both subfamilies. Diploid species had always one m pair carrying 5S rDNA genes, with pericentromeric location in different chromosome pairs. In the tetraploid cytotype of M. patagonica, the 5S rDNA probe hybridized to two pairs. The 2C DNA content obtained by FC varied twofold (from 1.85 to 2.52 pg), with significant differences between species. Mean chromosome length, karyotype formula, percentage of heterochromatin position of 5S rDNA locus, and nuclear Cx DNA content vary among Maihuenia and Pereskia species and allowed to differentiate them. Both genera are closely related and that the differences found are not strong enough to separate Maihuenioideae from Pereskioideae. | - |
| dc.description | Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; | - |
| dc.description | Fil: Urdampilleta, Juan Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; | - |
| dc.description | Fil: López- Carro, B.. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay; | - |
| dc.description | Fil: Santiñaque, F.. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay; | - |
| dc.description | Fil: Kiesling, Roberto. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Mendoza. Instituto Arg de Invest. de Las Zonas Aridas; Argentina; | - |
| dc.description | Fil: BERNARDELLO, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Springer Wien | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00606-013-0903-x | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00606-013-0903-x | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Karyotype | - |
| dc.subject | Heterochromatin | - |
| dc.subject | Physical mapping | - |
| dc.subject | C-DNA | - |
| dc.subject | Pereskia | - |
| dc.subject | Maihuenia | - |
| dc.subject | Ciencias de las Plantas, Botánica | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Classical and molecular cytogenetics and DNA content in Maihuenia and Pereskia (Cactaceae) | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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