Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Biancalana, Florencia | - |
| dc.creator | Kopprio, Germán Adolfo | - |
| dc.creator | Lara, Ruben Jose | - |
| dc.creator | Alonso, Cecilia | - |
| dc.date | 2017-12-21T17:01:21Z | - |
| dc.date | 2017-12-21T17:01:21Z | - |
| dc.date | 2017-07 | - |
| dc.date | 2017-12-12T19:41:28Z | - |
| dc.date.accessioned | 2019-04-29T15:50:32Z | - |
| dc.date.available | 2019-04-29T15:50:32Z | - |
| dc.date.issued | 2017-07 | - |
| dc.identifier | Alonso, Cecilia; Lara, Ruben Jose; Kopprio, Germán Adolfo; Biancalana, Florencia; A protocol for the simultaneous identification of chitin-containing particles and their associated bacteria; Elsevier Gmbh; Systematic And Applied Microbiology; 40; 5; 7-2017; 314-320 | - |
| dc.identifier | 0723-2020 | - |
| dc.identifier | http://hdl.handle.net/11336/31225 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303463 | - |
| dc.description | Chitin is the second most abundant polymer on Earth, playing a crucial role in the biogeochemical cycles. A core issue for studying its processing in aquatic systems is the identification and enumeration of chitin-containing particles and organisms, ideally in a manner that can be directly linked to bulk chitin quantification. The aim of this study was the development of such a technique. We successfully combined the methodology of bulk chitin determination using wheat germ agglutinin (FITC-WGA) for staining chitin-containing particles and organisms along with CARD-FISH staining of either chitin-containing eukaryotic cells or bacteria associated with them. Environmental chitin staining was successfully applied to natural water samples. Fungal hyphae, diatoms, and dinoflagellates, sestonic aggregates and chitin-containing structures derived from metazoa were observed. Also, hybridized bacteria attached to chitinaceous debris were clearly visualized. Finally, as proof of principle, cultured yeast cells were simultaneously-targeted by FITC-WGA and the EUK516 probe without exhibiting any interference between both stains. The presented approach appears as a powerful tool to evaluate the contribution of different size classes and organisms to chitin production and consumption, opening the possibility for application of single-cell approaches targeting the ecophysiology of chitin transformations in aquatic systems. | - |
| dc.description | Fil: Biancalana, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina | - |
| dc.description | Fil: Kopprio, Germán Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina | - |
| dc.description | Fil: Lara, Ruben Jose. Zentrum Fur Marine Tropenokologie Bremen; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina | - |
| dc.description | Fil: Alonso, Cecilia. Universidad de la República; Uruguay | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Gmbh | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S072320201730067X | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.syapm.2017.05.004 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | BACTERIA | - |
| dc.subject | CARD-FISH | - |
| dc.subject | CHITIN | - |
| dc.subject | SESTONIC AGGREGATES | - |
| dc.subject | WGA | - |
| dc.subject | Meteorología y Ciencias Atmosféricas | - |
| dc.subject | Ciencias de la Tierra y relacionadas con el Medio Ambiente | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | A protocol for the simultaneous identification of chitin-containing particles and their associated bacteria | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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