Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Reyna, Luciana | - |
| dc.creator | Flores Martín, Jésica Belén | - |
| dc.creator | Ridano, Magali Evelin | - |
| dc.creator | Panzetta-Dutari, Graciela Maria del Valle | - |
| dc.creator | Genti de Raimondi, Susana | - |
| dc.date | 2018-06-06T18:36:30Z | - |
| dc.date | 2018-06-06T18:36:30Z | - |
| dc.date | 2017-04 | - |
| dc.date | 2018-05-29T20:57:03Z | - |
| dc.date.accessioned | 2019-04-29T15:51:01Z | - |
| dc.date.available | 2019-04-29T15:51:01Z | - |
| dc.date.issued | 2017-04 | - |
| dc.identifier | Reyna, Luciana; Flores Martín, Jésica Belén; Ridano, Magali Evelin; Panzetta-Dutari, Graciela Maria del Valle; Genti de Raimondi, Susana; Chlorpyrifos induces endoplasmic reticulum stress in JEG-3 cells; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 40; 4-2017; 88-93 | - |
| dc.identifier | 0887-2333 | - |
| dc.identifier | http://hdl.handle.net/11336/47527 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303660 | - |
| dc.description | Chlorpyrifos (CPF) is an organophosphorous pesticide widely used in agricultural, industrial, and household applications. We have previously shown that JEG-3 cells are able to attenuate the oxidative stress induced by CPF through the adaptive activation of the Nrf2/ARE pathway. Considering that there is a relationship between oxidative stress and endoplasmic reticulum stress (ER), herein we investigated whether CPF also induces ER stress in JEG-3 cells. Cells were exposed to 50 μMor 100 μM CPF during 24 h in conditions where cell viability was not altered. Western blot and PCR assays were used to explore the protein and mRNA levels of ER stress biomarkers, respectively. CPF induced an increase of the typical ER stress-related proteins, such as GRP78/BiP and IRE1α, a sensor for the unfolded protein response, as well as in phospho-eIF2α and XBP1 mRNA splicing. Additionally,CPF led to a decrease in p53 protein expression. The downregulation of p53 levels induced by CPF was partially blocked when cells were exposed to CPF in the presence of the proteasome inhibitor MG132. Altogether, these findings point out that CPF induces ER stress in JEG-3 cells; however these cells are able to attenuate it downregulating the levels of the pro-apoptotic protein p53. | - |
| dc.description | Fil: Reyna, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Flores Martín, Jésica Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Ridano, Magali Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Panzetta-Dutari, Graciela Maria del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Genti de Raimondi, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Pergamon-Elsevier Science Ltd | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233316302582 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.tiv.2016.12.008 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | CHLORPYRIFOS | - |
| dc.subject | ENDOPLASMIC RETICULUM STRESS | - |
| dc.subject | P53 | - |
| dc.subject | JEG-3 CELLS | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Chlorpyrifos induces endoplasmic reticulum stress in JEG-3 cells | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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