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dc.creatorTrilling, Mirko-
dc.creatorBellora, Nicolás-
dc.creatorRutkowski, Andrzej J.-
dc.creatorde Graaf, Miranda-
dc.creatorDickinson, Paul-
dc.creatorRobertson, Kevin-
dc.creatorDa Costa, Olivia Prazeres-
dc.creatorGhazal, Peter-
dc.creatorFriedel, Caroline C.-
dc.creatorAlbà, M. Mar-
dc.creatorDölken, Lars-
dc.date2016-07-29T16:45:43Z-
dc.date2016-07-29T16:45:43Z-
dc.date2013-09-
dc.date2016-07-28T18:33:45Z-
dc.date.accessioned2019-04-29T15:51:24Z-
dc.date.available2019-04-29T15:51:24Z-
dc.date.issued2013-09-
dc.identifierTrilling, Mirko; Bellora, Nicolás; Rutkowski, Andrzej J.; de Graaf, Miranda; Dickinson, Paul; et al.; Deciphering the modulation of gene expression by type I and II interferons combining 4sU-tagging, translational arrest and in silico promoter analysis; Oxford University Press; Nucleic Acids Research; 41; 17; 9-2013; 8107-8125-
dc.identifier0305-1048-
dc.identifierhttp://hdl.handle.net/11336/6809-
dc.identifier1362-4962-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303845-
dc.descriptionInterferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement the combination of high-resolution gene-expression profiling of nascent RNA with translational inhibition of secondary feedback by cycloheximide. Unexpectedly, this approach revealed a prominent role of negative feedback mechanisms during the immediate (≤60 min) IFNα response. In contrast, a more complex picture involving both negative and positive feedback loops was observed on IFNγ treatment. IFNγ-induced repression of genes associated with regulation of gene expression, cellular development, apoptosis and cell growth resulted from cycloheximide-resistant primary IFNγ signalling. In silico promoter analysis revealed significant overrepresentation of SP1/SP3-binding sites and/or GC-rich stretches. Although signal transducer and activator of transcription 1 (STAT1)-binding sites were not overrepresented, repression was lost in absence of STAT1. Interestingly, basal expression of the majority of these IFNγ-repressed genes was dependent on STAT1 in IFN-naïve fibroblasts. Finally, IFNγ-mediated repression was also found to be evident in primary murine macrophages. IFN-repressed genes include negative regulators of innate and stress response, and their decrease may thus aid the establishment of a signalling perceptive milieu.-
dc.descriptionFil: Trilling, Mirko. Universitat Duisburg - Essen; Alemania-
dc.descriptionFil: Bellora, Nicolás. Parque de Investigación Biomédica de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina-
dc.descriptionFil: Rutkowski, Andrzej J.. University of Cambridge; Reino Unido-
dc.descriptionFil: de Graaf, Miranda. University of Cambridge; Reino Unido-
dc.descriptionFil: Dickinson, Paul. University Of Edinburgh; Reino Unido-
dc.descriptionFil: Robertson, Kevin. University Of Edinburgh; Reino Unido-
dc.descriptionFil: Da Costa, Olivia Prazeres. Universitat Technical Zu Munich; Alemania-
dc.descriptionFil: Ghazal, Peter. University Of Edinburgh; Reino Unido-
dc.descriptionFil: Friedel, Caroline C.. Ludwig-Maximilians-University Munich; Alemania-
dc.descriptionFil: Albà, M. Mar. Institució Catalana de Recerca I Estudis Avancats; España. Parque de Investigación Biomédica de Barcelona; España-
dc.descriptionFil: Dölken, Lars. University of Cambridge; Reino Unido-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherOxford University Press-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/17/8107-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783172/-
dc.relationinfo:eu-repo/semantics/altIdentifier/pmid/PMC3783172-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/nar/gkt589-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkt589-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectinterferon-
dc.subject4sU-tagging-
dc.subjectbioinformatics-
dc.subjectexpression-
dc.subjectCiencias de la Información y Bioinformática-
dc.subjectCiencias de la Computación e Información-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.subjectMétodos de Investigación en Bioquímica-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.subjectBiología Celular, Microbiología-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleDeciphering the modulation of gene expression by type I and II interferons combining 4sU-tagging, translational arrest and in silico promoter analysis-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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