Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Gerhardt, Edileusa C. M. | - |
| dc.creator | Moure, Vivian R. | - |
| dc.creator | Souza, Andrey W. | - |
| dc.creator | Pedrosa, Fabio O. | - |
| dc.creator | Souza, Emanuel M. | - |
| dc.creator | Diacovich, Lautaro | - |
| dc.creator | Gramajo, Hugo Cesar | - |
| dc.creator | Huergo, Luciano F. | - |
| dc.date | 2018-07-28T00:22:03Z | - |
| dc.date | 2018-07-28T00:22:03Z | - |
| dc.date | 2017-06 | - |
| dc.date | 2018-07-26T14:00:34Z | - |
| dc.date.accessioned | 2019-04-29T15:51:33Z | - |
| dc.date.available | 2019-04-29T15:51:33Z | - |
| dc.date.issued | 2017-06 | - |
| dc.identifier | Gerhardt, Edileusa C. M.; Moure, Vivian R.; Souza, Andrey W.; Pedrosa, Fabio O.; Souza, Emanuel M.; et al.; Expression and purification of untagged Glnk proteins from actinobacteria; Leibniz Research Centre for Working Environment and Human Factors; Excli Journal; 16; 6-2017; 949-958 | - |
| dc.identifier | 1611-2156 | - |
| dc.identifier | http://hdl.handle.net/11336/53368 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303913 | - |
| dc.description | The PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been welldocumented in Gram-negative bacteria. However, there are very few reports describing the in vitro properties and function of PII derived from Gram-positive bacteria. Here we present the heterologous expression and efficientpurification protocols for untagged PII from three Actinobacteria of medical and biotechnological interest namely: Mycobacterium tuberculosis, Rhodococcus jostii and Streptomyces coelicolor. Circular dichroism and gel filtrationanalysis supported that the purified proteins are correctly folded. The purification protocol described here will facilitate biochemical studies and help to uncover the biochemical functions of PII proteins in Actinobacteria. | - |
| dc.description | Fil: Gerhardt, Edileusa C. M.. Universidade Federal do Paraná; Brasil | - |
| dc.description | Fil: Moure, Vivian R.. Universidade Federal do Paraná; Brasil | - |
| dc.description | Fil: Souza, Andrey W.. Universidade Federal do Paraná; Brasil | - |
| dc.description | Fil: Pedrosa, Fabio O.. Universidade Federal do Paraná; Brasil | - |
| dc.description | Fil: Souza, Emanuel M.. Universidade Federal do Paraná; Brasil | - |
| dc.description | Fil: Diacovich, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Gramajo, Hugo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Huergo, Luciano F.. Universidade Federal do Paraná; Brasil | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Leibniz Research Centre for Working Environment and Human Factors | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579400/ | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.excli.de/volume16.php | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.excli.de/vol16/Huergo_27062017_proof.pdf | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | PII PROTEINS | - |
| dc.subject | ACTINOBACTERIA | - |
| dc.subject | NITROGEN | - |
| dc.subject | METABOLISM | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Expression and purification of untagged Glnk proteins from actinobacteria | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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