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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Chiapella, Graciela | - |
| dc.creator | Flores Martín, Jésica Belén | - |
| dc.creator | Ridano, Magali Evelin | - |
| dc.creator | Reyna, Luciana | - |
| dc.creator | Magnarelli, Gladis Griselda | - |
| dc.creator | Panzetta-Dutari, Graciela Maria del Valle | - |
| dc.creator | Genti de Raimondi, Susana | - |
| dc.date | 2017-10-26T21:57:40Z | - |
| dc.date | 2017-10-26T21:57:40Z | - |
| dc.date | 2013-07 | - |
| dc.date | 2017-10-02T19:05:40Z | - |
| dc.date.accessioned | 2019-04-29T15:52:27Z | - |
| dc.date.available | 2019-04-29T15:52:27Z | - |
| dc.date.issued | 2013-07 | - |
| dc.identifier | Chiapella, Graciela; Flores Martín, Jésica Belén; Ridano, Magali Evelin; Reyna, Luciana; Magnarelli, Gladis Griselda; et al.; The organophosphate chlorpyrifos disturbs redox balance and triggers antioxidant defense mechanisms in JEG-3 cells; Elsevier; Placenta; 34; 9; 7-2013; 792-798 | - |
| dc.identifier | 0143-4004 | - |
| dc.identifier | http://hdl.handle.net/11336/27158 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/304304 | - |
| dc.description | Introduction: Reactive oxygen species (ROS) are produced by a number of physiological and pathological processes which influence the function of a diverse array of cellular events. An imbalance between ROS generation and elimination was reported for different environmental xenobiotics exposure. Here, we analyzed the effect of chlorpyrifos (CPF) on the JEG-3 cell antioxidant defense in conditions where cell viability and morphology were not altered. Methods: Acetylcholinesterase (AChE) activity, reduced glutathione (GSH) content and catalase (CAT) antioxidant enzyme activity were measured by biochemical studies. ROS production was detected using the fluorogenic probe 2′,7′-dichlorodihydrofluorescein diacetate. The transcript level of superoxide dismutase enzyme 1 (SOD1), glutathione reductase (GR), heme oxygenase-1 (HO-1), and nuclear factor E2-related factor 2 (Nrf2) as well as Nrf2 protein amount were analyzed by quantitative real time PCR and Western blot, respectively. Results: The results showed that CPF inhibited AChE activity, induced ROS production, upregulated CAT activity, and decreased GSH concentration. In response to CPF exposure, GR and HO-1 mRNA levels were increased with no changes in SOD1 mRNA. Furthermore, CPF significantly augmented Nrf2 at both mRNA and protein levels trigging the antioxidant status by increasing nuclear Nrf2 translocation. Discussion and conclusion: Taken together, these data indicate that JEG-3 cells are able to attenuate the oxidative stress induced by CPF through the adaptive activation of the Nrf2/ARE pathway. | - |
| dc.description | Fil: Chiapella, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Patagonia Norte. Instituto de Investigación y Des. En Ing. de Procesos, Biotecnología y Energias Alternativas. Idepa - Subsede San Antonio Oeste; Argentina | - |
| dc.description | Fil: Flores Martín, Jésica Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Ridano, Magali Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Reyna, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Magnarelli, Gladis Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Patagonia Norte. Instituto de Investigación y Des. En Ing. de Procesos, Biotecnología y Energias Alternativas. Idepa - Subsede San Antonio Oeste; Argentina | - |
| dc.description | Fil: Panzetta-Dutari, Graciela Maria del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Genti de Raimondi, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.placenta.2013.06.007 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0143400413002609 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Chlorpyrifos | - |
| dc.subject | Placenta | - |
| dc.subject | Antioxidant response | - |
| dc.subject | Nrf2 | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | The organophosphate chlorpyrifos disturbs redox balance and triggers antioxidant defense mechanisms in JEG-3 cells | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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