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dc.provenanceCONICET-
dc.creatorBaravalle, María Eugenia-
dc.creatorThompson, Carolina Soledad-
dc.creatorTorioni de Echaide, S.-
dc.creatorPalacios, Carlos Adolfo-
dc.creatorValentini, B.-
dc.creatorSuárez, C. E.-
dc.creatorChristensen, M. Florín-
dc.creatorEchaide, Ignacio Eduardo-
dc.date2019-02-06T22:52:52Z-
dc.date2019-02-06T22:52:52Z-
dc.date2010-12-
dc.date2019-01-29T18:09:03Z-
dc.date.accessioned2019-04-29T15:53:19Z-
dc.date.available2019-04-29T15:53:19Z-
dc.date.issued2010-12-
dc.identifierBaravalle, María Eugenia; Thompson, Carolina Soledad; Torioni de Echaide, S.; Palacios, Carlos Adolfo; Valentini, B.; et al.; The novel protein BboRhop68 is expressed by intraerythrocytic stages of Babesia bovis; Elsevier Ireland; Parasitology International; 59; 4; 12-2010; 571-578-
dc.identifier1383-5769-
dc.identifierhttp://hdl.handle.net/11336/69615-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/304698-
dc.descriptionThe apical complex of intracellular hemoparasites contains organelles like micronemes and rhoptries, specialized structures required for adherence and invasion of host cells. Several molecules discharged from rhoptries have been identified from Plasmodium spp., but only a single rhoptry associated protein-1 (RAP-1) has been characterized from Babesia bovis. In silico search of the B. bovis genome allowed to identifying a sequence homologous to the gene that encodes a P. falciparum rhoptry protein PfRhop148. The intron-less 1830. bp novel gene, predicted a 68. kDa protein, and it was highly conserved among different B. bovis strains and isolates. The deducted protein from the B. bovis T2Bo strain, named BboRhop68, showed two putative transmembrane domains, at least seven B-cell epitopes, and a well conserved DUF501 super family domain. The bborhop68 gene was amplified, analyzed and compared among different B. bovis strains and isolates showing overall high sequence conservation. A fragment of bborhop68 was expressed as a recombinant fusion protein (rBboRhop68). The mice anti-rBboRhop68 serum identified the novel protein in intraerythrocytic trophozoites and merozoites by WB and ELISA, but not in free merozoites. Sera from naturally and experimentally infected bovines also recognized BboRhop68, suggesting that it is expressed and immunogenic during B. bovis infection. Fluorescence microscopy analysis using anti-rBboRhop68 antibodies showed a rod structure associated to trophozoites and merozoites infected erythrocytes, but this pattern of reactivity was not observed in free merozoites. The BboRhop68 was also not detected in ELISA based on solubilized merozoites. Thus, at least three independent lines of evidence support differential expression of BboRhop68 in intraerythrocytic stages of B. bovis and its possible functional role immediately after B. bovis erythrocyte invasion. The results of this work suggest that BboRhop68 could be considered as a novel additional target for developing improved methods to control bovine babesiosis.-
dc.descriptionFil: Baravalle, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina-
dc.descriptionFil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina-
dc.descriptionFil: Torioni de Echaide, S.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina-
dc.descriptionFil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina-
dc.descriptionFil: Valentini, B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina-
dc.descriptionFil: Suárez, C. E.. United States Department of Agriculture. Agricultural Research Service; Argentina-
dc.descriptionFil: Christensen, M. Florín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina-
dc.formatapplication/pdf-
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dc.languageeng-
dc.publisherElsevier Ireland-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383576910001315-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.parint.2010.07.008-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/69615-
dc.subjectAPICOMPLEXA-
dc.subjectBABESIA BOVIS-
dc.subjectBBORHOP68-
dc.subjectRHOPTRY-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleThe novel protein BboRhop68 is expressed by intraerythrocytic stages of Babesia bovis-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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