Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Álvarez Paggi, Damián Jorge | - |
| dc.creator | Castro, Maria Ana | - |
| dc.creator | Tortora, Verónica | - |
| dc.creator | Castro, Laura | - |
| dc.creator | Radi, Rafael | - |
| dc.creator | Murgida, Daniel Horacio | - |
| dc.date | 2015-06-16T18:59:04Z | - |
| dc.date | 2015-06-16T18:59:04Z | - |
| dc.date | 2013-03 | - |
| dc.date | 2016-03-30 10:35:44.97925-03 | - |
| dc.date.accessioned | 2019-04-29T15:54:49Z | - |
| dc.date.available | 2019-04-29T15:54:49Z | - |
| dc.identifier | Álvarez Paggi, Damián Jorge; Castro, Maria Ana; Tortora, Verónica; Castro, Laura; Radi, Rafael; et al.; Electrostatically Driven Second-Sphere Ligand Switch between High and Low Reorganization Energy Forms of Native Cytochrome c; Amer Chemical Soc; Journal of the American Chemical Society; 135; 3-2013; 4389-4397 | - |
| dc.identifier | 0002-7863 | - |
| dc.identifier | http://hdl.handle.net/11336/757 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/305204 | - |
| dc.description | We have employed a combination of protein film voltammetry, time-resolved vibrational spectroelectrochemistry and molecular dynamics simulations to evaluate the electron-transfer reorganization free energy (λ) of cytochrome c (Cyt) in electrostatic complexes that mimic some basic features of protein–protein and protein–lipid interactions. The results reveal the existence of two native-like conformations of Cyt that present significantly different λ values. Conversion from the high to the low λ forms is triggered by electrostatic interactions, and involves the rupture of a weak H-bond between first- (M80) and second-sphere (Y67) ligands of the heme iron, as a distinctive feature of the conformational switch. The two flexible Ω loops operate as transducers of the electrostatic signal. This fine-tuning effect is abolished in the Y67F Cyt mutant, which presents a λ value similar to the WT protein in electrostatic complexes. We propose that interactions of Cyt with the natural redox partner proteins activate a similar mechanism to minimize the reorganization energy of interprotein electron transfer. | - |
| dc.description | Fil: Álvarez Paggi, Damián Jorge. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina; | - |
| dc.description | Fil: Castro, Maria Ana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina; | - |
| dc.description | Fil: Tortora, Verónica. Universidad de la República. Departamento de Bioquímica and Center for Free Radical and Biomedical Research; Uruguay; | - |
| dc.description | Fil: Castro, Laura. Universidad de la República. Departamento de Bioquímica and Center for Free Radical and Biomedical Research; Uruguay; | - |
| dc.description | Fil: Radi, Rafael. Universidad de la República. Departamento de Bioquímica and Center for Free Radical and Biomedical Research; Uruguay; | - |
| dc.description | Fil: Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina; | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Amer Chemical Soc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/dx.doi.org/10.1021/ja311786b | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ja311786b | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | cytochrome c | - |
| dc.subject | electron transfer | - |
| dc.subject | reorganization energy | - |
| dc.subject | electrostatic switch | - |
| dc.subject | Ciencias Naturales y Exactas | - |
| dc.subject | Ciencias Químicas | - |
| dc.subject | Físico-química, Ciencia de Los Polímeros, Electroquímica | - |
| dc.title | Electrostatically Driven Second-Sphere Ligand Switch between High and Low Reorganization Energy Forms of Native Cytochrome c | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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