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dc.creatorIrazusta, Verónica Patricia-
dc.creatorEstévez, Maria Cristina-
dc.creatorAmoroso, Maria Julia del R.-
dc.creatorCastellanos, Lucia Ines-
dc.date2018-03-05T19:22:12Z-
dc.date2018-03-05T19:22:12Z-
dc.date2012-06-
dc.date2018-03-02T14:57:38Z-
dc.date.accessioned2019-04-29T15:56:40Z-
dc.date.available2019-04-29T15:56:40Z-
dc.identifierIrazusta, Verónica Patricia; Estévez, Maria Cristina; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress; Springer; Biometals; 25; 3; 6-2012; 517-527-
dc.identifier0966-0844-
dc.identifierhttp://hdl.handle.net/11336/37863-
dc.identifier1572-8773-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/305892-
dc.descriptionIn order to understand the mechanism involved in Rhodotorula mucilaginosa RCL-11 resistance to copper a proteomic study was conducted. Atomic absorption spectroscopy showed that the copper concentration in the medium decreased from 0.5 to 0.19 mM 48 h after inoculation of the yeast. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed expression of differential bands between cells with and without copper. In order to study this difference, two-dimensional electrophoresis of R. mucilaginosa RCL-11 exposed to Cu for 16, 24, and 48 h was carried out. Identification of differentially expressed proteins was performed by MALDI-TOF/TOF. Ten of the 16 spots identified belonged to heat shock proteins. Superoxide dismutase, methionine synthase and beta-glucosidase were also found over-expressed at high copper concentrations. The results obtained in the present work show that when R. mucilaginosa RCL-11 is exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, are expressed.-
dc.descriptionFil: Irazusta, Verónica Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina-
dc.descriptionFil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina-
dc.descriptionFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina-
dc.descriptionFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10534-012-9531-0-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10534-012-9531-0-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectBIOREMEDIATION-
dc.subjectCOPPER OVERLOAD-
dc.subjectPROTEOMIC-
dc.subjectYEAST-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleProteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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