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dc.creatorMarin, Maia Solange-
dc.creatorHolani, Ravi-
dc.creatorShah, Chaitanya B.-
dc.creatorOdeón, Anselmo Carlos-
dc.creatorCobo, Eduardo Ruben-
dc.date2018-08-14T15:18:13Z-
dc.date2018-08-14T15:18:13Z-
dc.date2017-11-
dc.date2018-08-13T17:57:17Z-
dc.date.accessioned2019-04-29T15:56:53Z-
dc.date.available2019-04-29T15:56:53Z-
dc.identifierMarin, Maia Solange; Holani, Ravi; Shah, Chaitanya B.; Odeón, Anselmo Carlos; Cobo, Eduardo Ruben; Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium; Pergamon-Elsevier Science Ltd; Molecular Immunology; 91; 11-2017; 249-258-
dc.identifier0161-5890-
dc.identifierhttp://hdl.handle.net/11336/55356-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/305984-
dc.descriptionCathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases.-
dc.descriptionFil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Holani, Ravi. University of Calgary; Canadá-
dc.descriptionFil: Shah, Chaitanya B.. University of Calgary; Canadá-
dc.descriptionFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina-
dc.descriptionFil: Cobo, Eduardo Ruben. University of Calgary; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherPergamon-Elsevier Science Ltd-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.molimm.2017.09.011-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161589017305102-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectANTIMICROBIAL PEPTIDE-
dc.subjectCATHELICIDIN-
dc.subjectESCHERICHIA COLI-
dc.subjectINTESTINAL EPITHELIUM-
dc.subjectTOLL-LIKE RECEPTOR-
dc.subjectOtras Biotecnologías de la Salud-
dc.subjectBiotecnología de la Salud-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleCathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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