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dc.creatorRico-Pérez, Gadea-
dc.creatorPezza, Alejandro-
dc.creatorPucciarelli, M. Graciela-
dc.creatorde Pedro, Miguel A.-
dc.creatorSoncini, Fernando Carlos-
dc.creatorGarcía del Portillo, Francisco-
dc.date2018-07-17T19:38:39Z-
dc.date2018-07-17T19:38:39Z-
dc.date2016-02-
dc.date2018-07-17T13:56:11Z-
dc.date.accessioned2019-04-29T15:56:56Z-
dc.date.available2019-04-29T15:56:56Z-
dc.identifierRico-Pérez, Gadea; Pezza, Alejandro; Pucciarelli, M. Graciela; de Pedro, Miguel A.; Soncini, Fernando Carlos; et al.; A novel peptidoglycan D,L-endopeptidase induced by Salmonella inside eukaryotic cells contributes to virulence; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 99; 3; 2-2016; 546-556-
dc.identifier0950-382X-
dc.identifierhttp://hdl.handle.net/11336/52497-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/306003-
dc.descriptionBacteria remodel peptidoglycan structure in response to environmental changes. Many enzymes are involved in peptidoglycan metabolism; however, little is known about their responsiveness in a defined environment or the modes they assist bacteria to adapt to new niches. Here, we focused in peptidoglycan enzymes that intracellular bacterial pathogens use inside eukaryotic cells. We identified a peptidoglycan enzyme induced by Salmonella enterica serovar Typhimurium in fibroblasts and epithelial cells. This enzyme, which shows γ-D-glutamyl-meso-diaminopimelic acid D,L-endopeptidase activity, is also produced by the pathogen in media with limited nutrients and in resting conditions. The enzyme, termed EcgA for endopeptidase responding to cessation of growth', is encoded in a S. Typhimurium genomic island absent in Escherichia coli. EcgA production is strictly dependent on the virulence regulator PhoP in extra- and intracellular environments. Consistent to this regulation, a mutant lacking EcgA is attenuated in the mouse typhoid model. These findings suggest that specialised peptidoglycan enzymes, such as EcgA, might facilitate Salmonella adaptation to the intracellular lifestyle. Moreover, they indicate that readjustment of peptidoglycan metabolism inside the eukaryotic cell is essential for host colonisation. Many enzymes direct peptidoglycan metabolism but little it is known about their regulation.-
dc.descriptionFil: Rico-Pérez, Gadea. Consejo Superior de Investigaciones Científicas; España-
dc.descriptionFil: Pezza, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina-
dc.descriptionFil: Pucciarelli, M. Graciela. Consejo Superior de Investigaciones Científicas; España-
dc.descriptionFil: de Pedro, Miguel A.. Consejo Superior de Investigaciones Científicas; España-
dc.descriptionFil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina-
dc.descriptionFil: García del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherWiley Blackwell Publishing, Inc-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/mmi.13248-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.13248-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectSalmonella-
dc.subjecthost colonisation-
dc.subjectpeptidoglycan metabolism-
dc.subjectPhoP-regulated-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleA novel peptidoglycan D,L-endopeptidase induced by Salmonella inside eukaryotic cells contributes to virulence-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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