Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Lepanto, Paola | - |
| dc.creator | Lecumberry, Federico | - |
| dc.creator | Rossello, Jéssica | - |
| dc.creator | Kierbel, Arlinet Verónica | - |
| dc.date | 2018-01-04T19:17:34Z | - |
| dc.date | 2018-01-04T19:17:34Z | - |
| dc.date | 2014-02 | - |
| dc.date | 2018-01-03T19:03:51Z | - |
| dc.date.accessioned | 2019-04-29T15:57:10Z | - |
| dc.date.available | 2019-04-29T15:57:10Z | - |
| dc.identifier | Kierbel, Arlinet Verónica; Rossello, Jéssica; Lecumberry, Federico; Lepanto, Paola; A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells; Academic Press Ltd-elsevier Science Ltd; Molecular And Cellular Probes; 28; 1; 2-2014; 1-5 | - |
| dc.identifier | 0890-8508 | - |
| dc.identifier | http://hdl.handle.net/11336/32351 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/306088 | - |
| dc.description | Formation of multicellular structures such as biofilms is an important feature in the physiopathology of many disease-causing bacteria. We recently reported that Pseudomonas aeruginosa adheres to epithelial cells rapidly forming early biofilm-like aggregates, which can then be internalized into cells. Conventional methods to measure adhesion/internalization, such as dilution plating for total cell-associated or antibiotic protected bacteria, do not distinguish between single and aggregated bacteria. We report a procedure that combining double bacteria labeling, confocal microscopy and image analysis allows identification and quantification of the number of adhered and internalized bacteria distinguishing between single and aggregated bacterial cells. A plugin for Fiji to automatically perform these procedures has been generated. | - |
| dc.description | Fil: Lepanto, Paola. Instituto Pasteur de Montevideo; Uruguay | - |
| dc.description | Fil: Lecumberry, Federico. Universidad de la República. Facultad de Ciencias; Uruguay | - |
| dc.description | Fil: Rossello, Jéssica. Instituto Pasteur de Montevideo; Uruguay | - |
| dc.description | Fil: Kierbel, Arlinet Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Academic Press Ltd-elsevier Science Ltd | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mcp.2013.10.001 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0890850813000534 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Pseudomonas aeruginosa | - |
| dc.subject | Adhesion | - |
| dc.subject | Internalization | - |
| dc.subject | Multicellular structures | - |
| dc.subject | Image analysis | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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