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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Comisión de Investigaciones Científicas | - |
| dc.contributor | Blanco, F.A. | - |
| dc.contributor | Zanetti, M.E. | - |
| dc.contributor | Daleo, Gustavo Raúl | - |
| dc.creator | Blanco, F.A. | - |
| dc.creator | Zanetti, M.E. | - |
| dc.creator | Daleo, Gustavo Raúl | - |
| dc.date | 2008-01-30 | - |
| dc.date.accessioned | 2019-04-29T16:12:54Z | - |
| dc.date.available | 2019-04-29T16:12:54Z | - |
| dc.date.issued | 2008-01-30 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/5606 | - |
| dc.identifier | Recurso Completo | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/311764 | - |
| dc.description | Plant response to pathogens involves an intricate network of signal transduction pathways. Here, potato cell cultures were used to study signal transduction in response to elicitors from<em>Phytophthora infestans</em>. Pretreatment of cells with Ser/Thr protein kinase inhibitors, EGTA, calmodulin antagonists or a channel blocker abolished the induction of two enzymes involved in defence responses, phenylalanine ammonia-lyase (PAL) and peroxidase. Phosphatase inhibitors caused an increase of these activities in the absence of elicitors. Hyphal cell wall components (HWC) from an incompatible race (HWC 0) produced a rapid and transient increment of histone phosphorylation, whereas induction by HWC from a compatible race (HWC C) was less pronounced and more sustained. As activities were calcium-dependent, a fraction enriched in calcium-dependent protein kinases (CDPKs) was obtained by DEAE chromatography. Fractions from HWC 0- and HWC C-treated cells presented higher kinase activity than that from untreated cells. Moreover, total activity was higher in the incompatible than in the compatible interaction. Activity was calcium-dependent, partially inhibited by calmodulin antagonists and able to phosphorylate syntide-2, a specific substrate of CDPKs. An in-gel kinase assay showed the presence of a band of approximately 50kDa whose activity was higher in HWC 0- than in HWC C-treated cells and was not detected in control extracts. This report presents evidences of the differential activation of CDPKs in response to elicitors from different races of<em>P. infestans</em>, revealing that these protein kinases participate in the defence response to oomycete. | - |
| dc.format | application/pdf | - |
| dc.format | 9 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/5606 | - |
| dc.source.uri | Recurso Completo | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.title | Calcium-dependent Protein Kinases are Involved in Potato Signal Transduction. | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Comisión de Investigaciones Científicas de la Prov. de Buenos Aires | |
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