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dc.provenanceComisión de Investigaciones Científicas-
dc.contributorCortizo, Ana María-
dc.contributorSedlinsky, Claudia-
dc.contributorMcCarthy, Antonio Desmond-
dc.contributorBlanco, Alcira-
dc.contributorSchurman, León-
dc.creatorCortizo, Ana María-
dc.creatorSedlinsky, Claudia-
dc.creatorMcCarthy, Antonio Desmond-
dc.creatorBlanco, Alcira-
dc.creatorSchurman, León-
dc.date2006-
dc.date.accessioned2019-04-29T16:13:03Z-
dc.date.available2019-04-29T16:13:03Z-
dc.date.issued2006-
dc.identifierhttp://digital.cic.gba.gob.ar/handle/11746/4418-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/311817-
dc.descriptionAn association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.-
dc.formatapplication/pdf-
dc.format9 p.-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightsAttribution 4.0 International (BY 4.0)-
dc.sourcereponame:CIC Digital (CICBA)-
dc.sourceinstname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires-
dc.sourceinstacron:CICBA-
dc.source.urihttp://digital.cic.gba.gob.ar/handle/11746/4418-
dc.subjectCiencias Químicas-
dc.titleOsteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/submittedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: Comisión de Investigaciones Científicas de la Prov. de Buenos Aires

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