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dc.provenanceComisión de Investigaciones Científicas-
dc.contributorCaro, Luis A.-
dc.contributorSantecchia, Natalia-
dc.contributorMarinangeli, Pablo-
dc.contributorCurvetto, Néstor-
dc.contributorHernández, Luis Francisco-
dc.creatorCaro, Luis A.-
dc.creatorSantecchia, Natalia-
dc.creatorMarinangeli, Pablo-
dc.creatorCurvetto, Néstor-
dc.creatorHernández, Luis Francisco-
dc.date2003-
dc.date.accessioned2019-04-29T16:13:16Z-
dc.date.available2019-04-29T16:13:16Z-
dc.date.issued2003-
dc.identifierhttp://digital.cic.gba.gob.ar/handle/11746/4353-
dc.identifierDocumento completo-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/311901-
dc.descriptionThe induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned medium, but with 0.15 mgL-1 of BAP. After 2 months, microshoots with 2-3 leaves were selected and cultured in BTMm-agar, supplemented with 5 mg.L-1 IBA or in liquid BTMm on perlite and, in the presence or absence of A. rhizogenes (Ar) and in combination with 3 mg.L-1 IBA. Rooting in P. chilensis reached 100.0% when Ar infection was produced in the presence of IBA, increasing both, the number and dry weight of roots. In N. alpina, 90.0% of rooting efficiency was obtained when Ar infection was produced in liquid culture and in the absence of auxin.-
dc.formatapplication/pdf-
dc.formatp. 311-318-
dc.languageeng-
dc.publisherInstituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM-CONICET)-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightsAttribution-NonCommercial 4.0 International (BY-NC 4.0)-
dc.sourcereponame:CIC Digital (CICBA)-
dc.sourceinstname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires-
dc.sourceinstacron:CICBA-
dc.source.urihttp://digital.cic.gba.gob.ar/handle/11746/4353-
dc.source.uriDocumento completo-
dc.subjectBiología Celular, Microbiología-
dc.titleAgrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: Comisión de Investigaciones Científicas de la Prov. de Buenos Aires

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