Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Comisión de Investigaciones Científicas | - |
| dc.contributor | Fernandez, María Belén | - |
| dc.contributor | Daleo, Gustavo Raúl | - |
| dc.contributor | GuevaRA, María Gabriela | - |
| dc.creator | Fernandez, María Belén | - |
| dc.creator | Daleo, Gustavo Raúl | - |
| dc.creator | GuevaRA, María Gabriela | - |
| dc.date | 2015-01-20 | - |
| dc.date.accessioned | 2019-04-29T16:13:54Z | - |
| dc.date.available | 2019-04-29T16:13:54Z | - |
| dc.date.issued | 2015-01-20 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/5361 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/312143 | - |
| dc.description | Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during <em>Solanum tuberosum- Phytophthora infestans</em> interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with <em>P. infestans.</em> Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from <em>S. tuberosum </em>(Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- <em>P. infestans</em> interaction, resulting in the restriction of the pathogen spread during plantepathogen interaction. | - |
| dc.format | application/pdf | - |
| dc.format | 10 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/5361 | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.title | Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3) | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Comisión de Investigaciones Científicas de la Prov. de Buenos Aires | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.