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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Comisión de Investigaciones Científicas | - |
| dc.contributor | Dinolfo, María Inés | - |
| dc.contributor | Barros, Germán G. | - |
| dc.contributor | Stenglein, Sebastián | - |
| dc.creator | Dinolfo, María Inés | - |
| dc.creator | Barros, Germán G. | - |
| dc.creator | Stenglein, Sebastián | - |
| dc.date | 2012 | - |
| dc.date.accessioned | 2019-04-29T16:14:06Z | - |
| dc.date.available | 2019-04-29T16:14:06Z | - |
| dc.date.issued | 2012 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/6437 | - |
| dc.identifier | Recurso Completo | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/312207 | - |
| dc.description | <em>Fusarium</em>species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of<em>Fusarium poae</em>has increased within the<em>Fusarium</em>head blight complex.<em>Fusarium poae</em>is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the<em>tri7</em>gene to detect potential nivalenol-producing<em>F. poae</em>isolates. A total of 125<em>F. poae</em>, four<em>F. cerealis</em>, two<em>F. culmorum</em>, one<em>F. langsethiae</em>, one<em>F. sporotrichioides</em>and seven<em>F. graminearum</em>, plus<em>F. austroamericanum, F. meridionale, F. graminearum sensu stricto</em>and<em>F. cortaderiae</em>from the NRRL collection were analysed, and only<em>F. poae</em>isolates gave a positive result for the presence of a 296-bp partial<em>tri7</em>DNA fragment. Moreover, the primer set was tested from cereal seed samples where<em>F. poae</em>and other<em>Fusarium</em>species with a negative result for the specific reaction (<em>F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti</em>and<em>F. acuminatum</em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing<em>F. poae</em>isolates. | - |
| dc.format | application/pdf | - |
| dc.format | 6 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution 4.0 International (BY 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/6437 | - |
| dc.source.uri | Recurso Completo | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.title | Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Comisión de Investigaciones Científicas de la Prov. de Buenos Aires | |
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