Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | INTA | - |
| dc.contributor | Llauger, Gabriela | - |
| dc.contributor | De Haro, Luis Alejandro | - |
| dc.contributor | Alfonso, Victoria | - |
| dc.contributor | Del Vas, Mariana | - |
| dc.creator | Llauger, Gabriela | - |
| dc.creator | De Haro, Luis Alejandro | - |
| dc.creator | Alfonso, Victoria | - |
| dc.creator | Del Vas, Mariana | - |
| dc.date | 2017-08-11T13:05:10Z | - |
| dc.date | 2017-08-11T13:05:10Z | - |
| dc.date | 2017 | - |
| dc.date.accessioned | 2019-04-29T16:26:23Z | - |
| dc.date.available | 2019-04-29T16:26:23Z | - |
| dc.date.issued | 2017-08-11T13:05:10Z | - |
| dc.date.issued | 2017-08-11T13:05:10Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier | 0168-1702 | - |
| dc.identifier | https://doi.org/10.1016/j.virusres.2017.01.002 | - |
| dc.identifier | http://hdl.handle.net/20.500.12123/954 | - |
| dc.identifier | http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313262 | - |
| dc.description | Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified interactionsof non-structural proteins P6/P6, P9-2/P9-2 and P6/P9-1. P9-1 and P6 are the major and minor compo-nents of the viroplasms respectively, whereas P9-2 is an N-glycosylated membrane protein of unknownfunction. Interactions involving P6 and P9-1 were confirmed by bimolecular fluorescence complementa-tion (BiFC) in rice protoplasts. We demonstrated that a region including a predicted coiled-coil domainwithin the C-terminal moiety of P6 was necessary for P6/P6 and P6/P9-1 interactions. In turn, a shortC-terminal arm was necessary for the previously reported P9-1 self-interaction. Transient expression ofthese proteins by agroinfiltration of Nicotiana benthamiana leaves showed very low accumulation levelsand further in silico analyses allowed us to identify conserved PEST degradation sequences [rich in pro-line (P), glutamic acid (E), serine (S), and threonine (T)] within P6 and P9-1. The removal of these PESTsequences resulted in a significant increase of the accumulation of both proteins. | - |
| dc.description | Inst. de Biotecnología | - |
| dc.description | Fil: Llauger, GabrielaINTA. Instituto de Biotecnología; Argentina | - |
| dc.description | Fil: De Haro, Luis Alejandro. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Alfonso, Victoria. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Del Vas, Mariana. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.source | Virus Research 230 :19-28. (2017) | - |
| dc.source | reponame:INTA Digital (INTA) | - |
| dc.source | instname:Instituto Nacional de Tecnología Agropecuaria | - |
| dc.source | instacron:INTA | - |
| dc.source.uri | 0168-1702 | - |
| dc.source.uri | https://doi.org/10.1016/j.virusres.2017.01.002 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/954 | - |
| dc.source.uri | http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub | - |
| dc.source.uri | https://doi.org/10.1016/j.virusres.2017.01.002 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/954 | - |
| dc.source.uri | http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub | - |
| dc.subject | Fitomejoramiento | - |
| dc.subject | Enfermedades de las Plantas | - |
| dc.subject | Virus de las Plantas | - |
| dc.subject | Identificación | - |
| dc.subject | Plant Breeding | - |
| dc.subject | Plant Diseases | - |
| dc.subject | Plant viruses | - |
| dc.subject | Identification | - |
| dc.subject | Mal de Río Cuarto | - |
| dc.title | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | INTA | |
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