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dc.provenanceINTA-
dc.contributorMaroniche, Guillermo Andrés-
dc.contributorGarcia, Julia Elena-
dc.contributorSalcedo, Florencia-
dc.contributorCreus, Cecilia M.-
dc.creatorMaroniche, Guillermo Andrés-
dc.creatorGarcia, Julia Elena-
dc.creatorSalcedo, Florencia-
dc.creatorCreus, Cecilia M.-
dc.date2017-08-24T13:50:00Z-
dc.date2017-08-24T13:50:00Z-
dc.date2017-
dc.date.accessioned2019-04-29T16:26:33Z-
dc.date.available2019-04-29T16:26:33Z-
dc.date.issued2017-08-24T13:50:00Z-
dc.date.issued2017-08-24T13:50:00Z-
dc.date.issued2017-
dc.identifier0944-5013-
dc.identifierhttps://doi.org/10.1016/j.micres.2016.11.009-
dc.identifierhttp://hdl.handle.net/20.500.12123/1032-
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0944501316307327?via%3Dihub-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313320-
dc.descriptionSince their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been sub-jected to intensive research due to their biotechnological potential as crop inoculants. Phylogeneticanalysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsis-tencies and low confidence often arise when working with close species. In this work, it was observedthat these difficulties might be explained by a high number of rRNA operons with considerable inter-genic variability within Azospirillum genomes. To search for alternative genetic markers from a list ofhousekeeping genes, the correlation between pairwise gene and whole-genome similarities was exam-ined. Due to its good performance, rpoD was selected for further analyses. Genus-specific primers for thePCR-amplification and sequencing of rpoD from Azospirillum spp. were designed and tested on 16 typestrains of different species. The sequences obtained were used for inferring a phylogenetic tree of thegenus, which was in turn used as a reference to successfully identify a collection of 31 azospirilla iso-lated from many different locations of Argentine. In addition, several strains that might represent novelspecies were detected. The results indicate that the sequencing of rpoD is a suitable alternative methodfor a confident molecular identification in Azospirillum spp.-
dc.descriptionInst. de Microbiología y Zoología Agrícola IMyZA-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourceMicrobiological Research 195 : 1-10 (January 2017)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri0944-5013-
dc.source.urihttps://doi.org/10.1016/j.micres.2016.11.009-
dc.source.urihttp://hdl.handle.net/20.500.12123/1032-
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0944501316307327?via%3Dihub-
dc.source.urihttps://doi.org/10.1016/j.micres.2016.11.009-
dc.source.urihttp://hdl.handle.net/20.500.12123/1032-
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0944501316307327?via%3Dihub-
dc.subjectAzospirillum-
dc.subjectMarcadores Genéticos-
dc.subjectRizobacterias-
dc.subjectGenotipos-
dc.subjectGenetic Markers-
dc.subjectRhizobacteria-
dc.subjectGenotypes-
dc.titleMolecular identification of Azospirillum spp.: limitations of 16S rRNA and qualities of rpoD as genetic markers-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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