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dc.provenanceINTA-
dc.contributorPontin, Mariela Ana-
dc.contributorPiccoli, Patricia Noemí-
dc.contributorBurba, Jose Luis-
dc.contributorBottini, Ambrosio Rubén-
dc.creatorPontin, Mariela Ana-
dc.creatorPiccoli, Patricia Noemí-
dc.creatorBurba, Jose Luis-
dc.creatorBottini, Ambrosio Rubén-
dc.date2017-09-07T12:46:16Z-
dc.date2017-09-07T12:46:16Z-
dc.date2015-07-
dc.date.accessioned2019-04-29T16:26:53Z-
dc.date.available2019-04-29T16:26:53Z-
dc.date.issued2017-09-07T12:46:16Z-
dc.date.issued2017-09-07T12:46:16Z-
dc.date.issued2015-07-
dc.identifier0031-9422 (Print)-
dc.identifier1873-3700 (Online)-
dc.identifierhttps://doi.org/10.1016/j.phytochem.2015.02.003-
dc.identifierhttp://hdl.handle.net/20.500.12123/1161-
dc.identifierhttp://ri.conicet.gov.ar/handle/11336/22405-
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313434-
dc.descriptionThis study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity-
dc.descriptionEEA La Consulta-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourcePhytochemistry 115 :152-160 (July 2015)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri0031-9422 (Print)-
dc.source.uri1873-3700 (Online)-
dc.source.urihttps://doi.org/10.1016/j.phytochem.2015.02.003-
dc.source.urihttp://hdl.handle.net/20.500.12123/1161-
dc.source.urihttp://ri.conicet.gov.ar/handle/11336/22405-
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub-
dc.source.urihttps://doi.org/10.1016/j.phytochem.2015.02.003-
dc.source.urihttp://hdl.handle.net/20.500.12123/1161-
dc.source.urihttp://ri.conicet.gov.ar/handle/11336/22405-
dc.source.urihttp://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub-
dc.subjectEcología de la planta-
dc.subjectAllium Sativum-
dc.subjectAjo-
dc.subjectTerpenoidos-
dc.subjectSclerotium Cepivorum-
dc.subjectPropiedades Antimicósicas-
dc.subjectGarlic-
dc.subjectTerpenoids-
dc.subjectAntifungal Properties-
dc.subjectTerpenos-
dc.titleAllium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
dc.coverageMendoza (province)-
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