Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | INTA | - |
| dc.contributor | Carloni, Edgardo José | - |
| dc.contributor | Ribotta, Andrea Noemi | - |
| dc.contributor | Lopez Colomba, Eliana | - |
| dc.contributor | Griffa, Sabrina Mariana | - |
| dc.contributor | Quiroga, Mariana Paola | - |
| dc.contributor | Tommasino, Exequiel Arturo | - |
| dc.contributor | Grunberg, Karina | - |
| dc.creator | Carloni, Edgardo José | - |
| dc.creator | Ribotta, Andrea Noemi | - |
| dc.creator | Lopez Colomba, Eliana | - |
| dc.creator | Griffa, Sabrina Mariana | - |
| dc.creator | Quiroga, Mariana Paola | - |
| dc.creator | Tommasino, Exequiel Arturo | - |
| dc.creator | Grunberg, Karina | - |
| dc.date | 2017-09-28T17:57:22Z | - |
| dc.date | 2017-09-28T17:57:22Z | - |
| dc.date | 2014-06 | - |
| dc.date.accessioned | 2019-04-29T16:27:23Z | - |
| dc.date.available | 2019-04-29T16:27:23Z | - |
| dc.date.issued | 2017-09-28T17:57:22Z | - |
| dc.date.issued | 2017-09-28T17:57:22Z | - |
| dc.date.issued | 2014-06 | - |
| dc.identifier | 0167-6857 | - |
| dc.identifier | 1573-5044 (Online version) | - |
| dc.identifier | https://doi.org/10.1007/s11240-014-0441-4 | - |
| dc.identifier | http://hdl.handle.net/20.500.12123/1353 | - |
| dc.identifier | https://link.springer.com/article/10.1007/s11240-014-0441-4 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313604 | - |
| dc.description | Buffel grass is a fodder grass that reproduces mainly via apomixis. Using in vitro tissue culture in apomictic species provides alternatives that can be applied in a breeding program. The aims of this work were to establish a protocol for in vitro generation of apomictic buffel grass genotypes using anthers as explants and to evaluate the genetic stability of regenerated plants via flow cytometry (FCM). Three genotypes were able to induce embryogenic calli in Murashige and Skoog (MS) medium supplemented with 6 mg/l of 2,4-dichlorophenoxyacetic acid. Seedling regeneration occurred in a MS medium supplemented with 0.5 mg/l napthaleneacetic acid + 1 mg/l 6-benzylaminopurin. Seedlings were derived from somatic embryos and the morphogenic process was induced using the somatic tissue of the stamens. Induction and regeneration efficiency depended on the genotype and was affected by date of tiller collection, different pretreatments or the interaction of these variables. FCM analyses in in vitro regenerated plants showed genetic instability in their nuclear DNA content. Plants with lower nuclear DNA content may indicate DNA aneuploids (8.6 %), whereas plants that had twice the value of nuclear DNA content (4.7 %) suggest in vitro polyploidization. This variation observed in apomictic genotypes provides an opportunity to obtain new variants, which may then be included as sources of genetic variability in buffel grass breeding programs. | - |
| dc.description | Fil: Carloni, Edgardo José. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Ribotta, Andrea Noemi. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Lopez Colomba, Eliana. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Griffa, Sabrina Mariana. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Quiroga, Mariana Paola. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Tommasino, Exequiel Arturo. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.description | Fil: Grunberg, Karina. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.source | Plant cell, tissue and organ culture 117 (3) : 311-322. (2014) | - |
| dc.source | reponame:INTA Digital (INTA) | - |
| dc.source | instname:Instituto Nacional de Tecnología Agropecuaria | - |
| dc.source | instacron:INTA | - |
| dc.source.uri | 0167-6857 | - |
| dc.source.uri | 1573-5044 (Online version) | - |
| dc.source.uri | https://doi.org/10.1007/s11240-014-0441-4 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/1353 | - |
| dc.source.uri | https://link.springer.com/article/10.1007/s11240-014-0441-4 | - |
| dc.source.uri | https://doi.org/10.1007/s11240-014-0441-4 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/1353 | - |
| dc.source.uri | https://link.springer.com/article/10.1007/s11240-014-0441-4 | - |
| dc.subject | Apomixis | - |
| dc.subject | Cultivo | - |
| dc.subject | Variación Somaclonal | - |
| dc.subject | ADN | - |
| dc.subject | Fitomejoramiento | - |
| dc.subject | Embrión Somático | - |
| dc.subject | Cultivation | - |
| dc.subject | Somaclonal Variation | - |
| dc.subject | DNA | - |
| dc.subject | Plant Breeding | - |
| dc.subject | Somatic Embryos | - |
| dc.title | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | INTA | |
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