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dc.provenanceINTA-
dc.contributorSetten, Lorena-
dc.contributorSoto, Gabriela Cinthia-
dc.contributorMozzicafreddo, Matteo-
dc.contributorFox, Ana Romina-
dc.contributorLisi, Christian Daniel-
dc.contributorCuccioloni, Massimiliano-
dc.contributorAngeletti, Mauro-
dc.contributorPagano, Elba Maria-
dc.contributorDiaz Paleo, Antonio Horacio-
dc.contributorAyub, Nicolás Daniel-
dc.creatorSetten, Lorena-
dc.creatorSoto, Gabriela Cinthia-
dc.creatorMozzicafreddo, Matteo-
dc.creatorFox, Ana Romina-
dc.creatorLisi, Christian Daniel-
dc.creatorCuccioloni, Massimiliano-
dc.creatorAngeletti, Mauro-
dc.creatorPagano, Elba Maria-
dc.creatorDiaz Paleo, Antonio Horacio-
dc.creatorAyub, Nicolás Daniel-
dc.date2017-10-10T14:32:22Z-
dc.date2017-10-10T14:32:22Z-
dc.date2013-05-13-
dc.date.accessioned2019-04-29T16:27:39Z-
dc.date.available2019-04-29T16:27:39Z-
dc.date.issued2017-10-10T14:32:22Z-
dc.date.issued2017-10-10T14:32:22Z-
dc.date.issued2013-05-13-
dc.identifier1932-6203-
dc.identifierhttps://doi.org/10.1371/journal.pone.0063666-
dc.identifierhttp://hdl.handle.net/20.500.12123/1450-
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313686-
dc.descriptionNitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.-
dc.descriptionFil: Setten, Lorena. INTA. Instituto de Genética "Ewald A. Favret"; Argentina-
dc.descriptionFil: Soto, Gabriela Cinthia. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Mozzicafreddo, Matteo. Università di Camerino. School of Biosciences and Biotechnology; Italia-
dc.descriptionFil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Lisi, Christian Daniel. INTA. Instituto de Genética "Ewald A. Favret"; Argentina-
dc.descriptionFil: Cuccioloni, Massimiliano. Università di Camerino. School of Biosciences and Biotechnology; Italia-
dc.descriptionFil: Angeletti, Mauro. Università di Camerino. School of Biosciences and Biotechnology; Italia-
dc.descriptionFil: Pagano, Elba Maria. INTA. Instituto de Genética "Ewald A. Favret"; Argentina-
dc.descriptionFil: Diaz Paleo, Antonio Horacio. INTA. Instituto de Genética "Ewald A. Favret"; Argentina-
dc.descriptionFil: Ayub, Nicolás Daniel. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.sourcePLoS ONE 8 (5) : e63666. (2013)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri1932-6203-
dc.source.urihttps://doi.org/10.1371/journal.pone.0063666-
dc.source.urihttp://hdl.handle.net/20.500.12123/1450-
dc.source.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666-
dc.source.urihttps://doi.org/10.1371/journal.pone.0063666-
dc.source.urihttp://hdl.handle.net/20.500.12123/1450-
dc.source.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666-
dc.subjectPseudomonas-
dc.subjectMicroorganismos-
dc.subjectOrganismos Modificados Genéticamente-
dc.subjectBacteria Fijadora del Nitrógeno-
dc.subjectFijación del Nitrógeno-
dc.subjectFijación del Amonio-
dc.subjectNitrogenasa-
dc.subjectRizosfera-
dc.subjectNitrógeno Asimilable-
dc.subjectInoculación-
dc.subjectInoculation-
dc.subjectArabidopsis-
dc.subjectBiotecnología Vegetal-
dc.subjectSustancias de Crecimiento Vegetal-
dc.subjectDeficiencias del Suelo-
dc.subjectMicroorganisms-
dc.subjectGenetically Modified Organisms-
dc.subjectNitrogen Fixing Bacteria-
dc.subjectNitrogen Fixation-
dc.subjectAmmonium Fixation-
dc.subjectNitrogenase-
dc.subjectRhizosphere-
dc.subjectDigestible Nitrogen-
dc.subjectPlant Biotechnology-
dc.subjectSoil Deficiencies-
dc.subjectArgentina-
dc.subjectDiazotrofos Endofitos-
dc.subjectBacterias Recombinantes-
dc.subjectPseudomonas Protogens Pf5-
dc.subjectFijación Biológica del Nitrógeno-
dc.subjectDeficiencias de Nitrógeno-
dc.titleEngineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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