1. RODNA
  2. INTA
  3. INTA
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.provenanceINTA-
dc.contributorPega, Juan Franco-
dc.contributorDescalzo, Adriana Maria-
dc.contributorRossetti, Luciana-
dc.contributorNanni, Mariana-
dc.contributorPerez, Carolina Daiana-
dc.contributorRizzo, Sergio Anibal-
dc.contributorDiaz, Gabriela Perez-
dc.creatorPega, Juan Franco-
dc.creatorDescalzo, Adriana Maria-
dc.creatorRossetti, Luciana-
dc.creatorNanni, Mariana-
dc.creatorPerez, Carolina Daiana-
dc.creatorRizzo, Sergio Anibal-
dc.creatorDiaz, Gabriela Perez-
dc.date2017-11-10T12:07:47Z-
dc.date2017-11-10T12:07:47Z-
dc.date2017-07-
dc.date.accessioned2019-04-29T16:28:35Z-
dc.date.available2019-04-29T16:28:35Z-
dc.date.issued2017-11-10T12:07:47Z-
dc.date.issued2017-11-10T12:07:47Z-
dc.date.issued2017-07-
dc.identifier0023-6438-
dc.identifierhttps://doi.org/10.1016/j.lwt.2017.03.010-
dc.identifierhttp://hdl.handle.net/20.500.12123/1742-
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S002364381730155X-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313911-
dc.descriptionIn this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or from fermented dairy foods manufactured with these microorganisms. These experimental set-ups involved two starter lactic acid bacteria (SLAB), Streptococcus thermophilus and Lactococcus lactis, as models across their most common technological environments: yoghurt, milk and cheese. The presence of free DNA modified qPCR results in levels sufficient to bias SLAB determinations, indicating that DNA may be a poor measure of cellular integrity. Furthermore, we showed for the first time that an order of magnitude more of free RNA than of DNA had to be used to modify RT-qPCR results. Importantly, the interference produced by free DNA and RNA was maintained across all the dairy matrices evaluated, independently of the SLAB and of the source of nucleic acids used, in both negative and positive samples. This study suggests that, contrary to DNA molecules, mRNA from dead microorganisms or from other extracellular sources may not be a determinant factor in skewing molecular quantifications. Therefore, our results support the potential use of mRNA as a molecular signature of cell integrity in dairy matrices.-
dc.descriptionInst. de Tecnol. de los Alimentos- ITA-
dc.descriptionFil: Pega, Juan Franco. INTA. Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Descalzo, Adriana Maria. INTA. Instituto Tecnología de Alimentos; Argentina. INTA-LABINTEX-Centre de Coopération Internationale en Recherche Agronomique pour le Déeveloppement (CIRAD), Déepartement PERSYST, UMR Qualisud; Francia-
dc.descriptionFil: Rossetti, Luciana. INTA. Instituto Tecnología de Alimentos; Argentina-
dc.descriptionFil: Perez, Carolina Daiana. INTA. Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Diaz, Gabriela Perez. INTA. Instituto Tecnología de Alimentos; Argentina-
dc.descriptionFil: Nanni, Mariana. INTA. Instituto Tecnología de Alimentos; Argentina-
dc.descriptionFil: Rizzo, Sergio Anibal. INTA. Instituto Tecnología de Alimentos; Argentina-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourceLWT - Food Science and Technology 80 : 416-422 (July 2017)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri0023-6438-
dc.source.urihttps://doi.org/10.1016/j.lwt.2017.03.010-
dc.source.urihttp://hdl.handle.net/20.500.12123/1742-
dc.source.urihttps://www.sciencedirect.com/science/article/pii/S002364381730155X-
dc.source.urihttps://doi.org/10.1016/j.lwt.2017.03.010-
dc.source.urihttp://hdl.handle.net/20.500.12123/1742-
dc.source.urihttps://www.sciencedirect.com/science/article/pii/S002364381730155X-
dc.subjectAcidos Nucléicos-
dc.subjectBacterias Acidolácticas-
dc.subjectMarcadores Genéticos-
dc.subjectProductos Lácteos-
dc.subjectNucleic Acids-
dc.subjectLactic Acid Bacteria-
dc.subjectGenetic Markers-
dc.subjectPCR-
dc.subjectMilk Products-
dc.subjectMarcadores Moleculares-
dc.subjectReacción en Cadena de la Polimerasa-
dc.titleImpact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: INTA

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem