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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Universidad de Buenos Aires. Facultad de Ciencias Económicas | - |
| dc.creator | Olivera, Noelia | - |
| dc.creator | Castuma, Celina E. | - |
| dc.creator | Hozbor, Daniela Flavia | - |
| dc.creator | Gaillard, María E. | - |
| dc.creator | Rumbo, Martín | - |
| dc.creator | Gómez, Ricardo M. | - |
| dc.date | 2014 | - |
| dc.date.accessioned | 2019-06-19T20:35:04Z | - |
| dc.date.available | 2019-06-19T20:35:04Z | - |
| dc.date.issued | 2014 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/7069 | - |
| dc.identifier | Recurso online | - |
| dc.identifier.uri | http://rodna.bn.gov.ar/jspui/handle/bnmm/326855 | - |
| dc.description | This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection. | - |
| dc.format | application/pdf | - |
| dc.format | 12 p. | - |
| dc.language | eng | - |
| dc.publisher | Hindawi Publishing Corporation | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution 4.0 International (BY 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/7069 | - |
| dc.source.uri | Recurso online | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.title | Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Facultad de Ciencias Económicas. UBA | |
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