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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Universidad Nacional de Rosario.RepHipUNR | - |
| dc.creator | Villar, Silvina Raquel | - |
| dc.creator | Ronco, María Teresa | - |
| dc.creator | Fernández Bussy, Rodrigo | - |
| dc.creator | Roggero, Eduardo | - |
| dc.creator | Lepletier, Ailin | - |
| dc.creator | Manarin, Romina | - |
| dc.creator | Savino, Wilson | - |
| dc.creator | Pérez, Ana Rosa | - |
| dc.creator | Bottasso, Oscar | - |
| dc.date | 2013-05-22 | - |
| dc.date | 2013-05-22 | - |
| dc.date.accessioned | 2019-07-15T18:43:42Z | - |
| dc.date.available | 2019-07-15T18:43:42Z | - |
| dc.date.issued | 2013-05-22 | - |
| dc.date.issued | 2013-05-22 | - |
| dc.identifier | Villar SR, Ronco MT, Ferna´ndez Bussy R, Roggero E, Lepletier A, et al. (2013) Tumor Necrosis Factor-a Regulates Glucocorticoid Synthesis in the Adrenal Glands of Trypanosoma cruzi Acutely-Infected Mice. The Role of TNF-R1. PLoS ONE 8(5): e63814. doi:10.1371/journal.pone.0063814 | - |
| dc.identifier | 1932-6203 | - |
| dc.identifier | http://hdl.handle.net/2133/3081 | - |
| dc.identifier | http://hdl.handle.net/2133/3081 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar/jspui/handle/bnmm/568220 | - |
| dc.description | Adrenal steroidogenesis is under a complex regulation involving extrinsic and intrinsic adrenal factors. TNF-α is an inflammatory cytokine produced in response to tissue injury and several other stimuli. We have previously demonstrated that TNF-R1 knockout (TNF-R1−/−) mice have a dysregulated synthesis of glucocorticoids (GCs) during Trypanosoma cruzi acute infection. Since TNF-α may influence GCs production, not only through the hypothalamus-pituitary axis, but also at the adrenal level, we now investigated the role of this cytokine on the adrenal GCs production. Wild type (WT) and TNF-R1−/− mice undergoing acute infection (Tc-WT and Tc-TNF-R1−/− groups), displayed adrenal hyperplasia together with increased GCs levels. Notably, systemic ACTH remained unchanged in Tc-WT and Tc-TNF-R1−/− compared with uninfected mice, suggesting some degree of ACTH-independence of GCs synthesis. TNF-α expression was increased within the adrenal gland from both infected mouse groups, with Tc-WT mice showing an augmented TNF-R1 expression. Tc-WT mice showed increased levels of P-p38 and P-ERK compared to uninfected WT animals, whereas Tc-TNF-R1−/− mice had increased p38 and JNK phosphorylation respect to Tc-WT mice. Strikingly, adrenal NF-κB and AP-1 activation during infection was blunted in Tc-TNF-R1−/− mice. The accumulation of mRNAs for steroidogenic acute regulatory protein and cytochrome P450 were significantly increased in both Tc-WT and Tc-TNF-R1−/− mice; being much more augmented in the latter group, which also had remarkably increased GCs levels. TNF-α emerges as a potent modulator of steroidogenesis in adrenocortical cells during T. cruzi infection in which MAPK pathways, NF-κB and AP-1 seem to play a role in the adrenal synthesis of pro-inflammatory cytokines and enzymes regulating GCs synthesis. These results suggest the existence of an intrinsic immune-adrenal interaction involved in the dysregulated synthesis of GCs during murine Chagas disease. | - |
| dc.description | Fil. Villar, Silvina R. Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil. Ronco, María Teresa. Instituto de Fisiología Experimental (IFISE-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Argentina | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | PLOS (Public Library of Science) | - |
| dc.relation | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063814 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.source | reponame:RepHipUNR (UNR) | - |
| dc.source | instname:Universidad Nacional de Rosario | - |
| dc.source | instacron:UNR | - |
| dc.source.uri | http://hdl.handle.net/2133/3081 | - |
| dc.subject | Trypanosoma cruzi | - |
| dc.subject | Parasitic diseases | - |
| dc.subject | MAPK signaling cascades | - |
| dc.subject | Inflammation | - |
| dc.subject | Adrenal glands | - |
| dc.subject | Cytokines | - |
| dc.subject | Enzyme regulation | - |
| dc.subject | Gene expression | - |
| dc.title | Tumor Necrosis Factor-α Regulates Glucocorticoid Synthesis in the Adrenal Glands of Trypanosoma cruzi Acutely-Infected Mice. The Role of TNF-R1 | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | artículo | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Universidad Nacional de Rosario. RepHipUNR | |
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