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dc.provenanceRIUNNE - Universidad Nacional del Nordeste-
dc.creatorDolce, Natalia Raquel-
dc.creatorHernández Ramírez, Fabiola-
dc.creatorGonzález Arnao, María Teresa-
dc.date2018-03-26-
dc.date.accessioned2020-02-17T15:35:02Z-
dc.date.available2020-02-17T15:35:02Z-
dc.date.issued2018-03-26-
dc.identifierDolce, N. R, Hernández Ramírez, F; González Arnao, M. T. (2019). Cryopreservation of vanilla (Vanilla planifolia) root-tips: a new alternative for in vitro long term storage of its germplasm. The Third International Symposium on Plant Cryopreservation 2018. Simposio llevado a cabo en Bangkok, Thailand.-
dc.identifierhttp://repositorio.unne.edu.ar/handle/123456789/9192-
dc.identifier.urihttp://rodna.bn.gov.ar/jspui/handle/bnmm/576913-
dc.descriptionFil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.-
dc.descriptionFil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.-
dc.descriptionFil: Hernández Ramírez, Fabiola. Universidad Veracruzana. Facultad de Ciencias Químicas; México.-
dc.descriptionFil: González Arnao, María Teresa. Universidad Veracruzana. Facultad de Ciencias Químicas; México.-
dc.descriptionVanilla planifolia (Orchidaceae) is the natural source of vanillin, which is the most widely appreciated flavor compound in the world. Although vanilla is cultivated throughout the tropics, their natural distribution areas are being severely reduced due to the anthropogenic impact. As a result, ex situ preservation actions are necessary to safeguard the threatened diversity of this species. Cryopreservation of vanilla shoot-tips has been previously reported using the droplet-vitrification technique. However, survival and plant regeneration following this protocol were low and little reproducible. In this study, we evaluated a new alternative for cryopreservation of vanilla germplasm by using root-tips as explants and following the droplet- vitrification protocol. Maximum survival (~60%) and further regeneration (~40%) were obtained by preconditioning root-tips isolated from in vitro propagated plants on semisolid MS with 0.3 M sucrose (1 day), exposing to loading solution consisting of 0.4 M sucrose + 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (60 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 placed on aluminum foils strips. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. rowth recovery and induction of buds were efficiently achieved by culturing cryostored root-tips on MS added with 1 mg L-1 KIN or BAP. Plant regeneration was achieved by transferring the induced buds to MS media. This protocol has great potential for long-term conservation of V. planifolia germplasm and of other vanilla relatives.-
dc.formatapplication/pdf-
dc.formatp. 203-210-
dc.languageeng-
dc.publisherDepartment of Plant Science, Faculty of Science, Mahidol University-
dc.relation10.17660/ActaHortic.2019.1234.27-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Argentina-
dc.source.urihttp://repositorio.unne.edu.ar/handle/123456789/9192-
dc.subjectPlant Cryopreservation-
dc.subjectVanilla planifolia-
dc.subjectVanilla germplasm-
dc.titleCryopreservation of vanilla (Vanilla planifolia) root-tips: A new alternative for in vitro long-term storage of its germplasm-
dc.typeinfo:eu-repo/semantics/conferenceObject-
dc.typeinfo:ar-repo/semantics/documento de conferencia-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: RIUNNE - Universidad Nacional del Nordeste

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