1. RODNA
  2. FCEN
  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorHerrmann, C.K.-
dc.contributorBukata, L.-
dc.contributorMelli, L.-
dc.contributorMarchesini, M.I.-
dc.contributorCaramelo, J.J.-
dc.contributorComerci, D.J.-
dc.creatorHerrmann, C.K.-
dc.creatorBukata, L.-
dc.creatorMelli, L.-
dc.creatorMarchesini, M.I.-
dc.creatorCaramelo, J.J.-
dc.creatorComerci, D.J.-
dc.date.accessioned2018-05-04T21:53:54Z-
dc.date.accessioned2018-05-28T15:47:45Z-
dc.date.available2018-05-04T21:53:54Z-
dc.date.available2018-05-28T15:47:45Z-
dc.date.issued2013-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68348-
dc.descriptionPhosphatidylcholine (PC), a common phospholipid of the eukaryotic cell membrane, is present in the cell envelope of the intracellular pathogen Brucella abortus, the etiological agent of bovine brucellosis. In this pathogen, the biosynthesis of PC proceeds mainly through the phosphatidylcholine synthase pathway; hence, it relies on the presence of choline in the milieu. These observations imply that B. abortus encodes an as-yet-unknown choline uptake system. Taking advantage of the requirement of choline uptake for PC synthesis, we devised a method that allowed us to identify a homologue of ChoX, the high-affinity periplasmic binding protein of the ABC transporter ChoXWV. Disruption of the choX gene completely abrogated PC synthesis at low choline concentrations in the medium, thus indicating that it is a high-affinity transporter needed for PC synthesis via the PC synthase (PCS) pathway. However, the synthesis of PC was restored when the mutant was incubated in media with higher choline concentrations, suggesting the presence of an alternative low-affinity choline uptake activity. By means of a fluorescence-based equilibrium- binding assay and using the kinetics of radiolabeled choline uptake, we show that ChoX binds choline with an extremely high affinity, and we also demonstrate that its activity is inhibited by increasing choline concentrations. Cell infection assays indicate that ChoX activity is required during the first phase of B. abortus intracellular traffic, suggesting that choline concentrations in the early and intermediate Brucella-containing vacuoles are limited. Altogether, these results suggest that choline transport and PC synthesis are strictly regulated in B. abortus. © 2013, American Society for Microbiology.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourceJ. Bacteriol. 2013;195(3):493-501-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_00219193_v195_n3_p493_Herrmann.pdf-
dc.subjectcholine-
dc.subjectChoX protein-
dc.subjectperiplasmic binding protein-
dc.subjectphosphatidylcholine-
dc.subjectunclassified drug-
dc.subjectarticle-
dc.subjectbinding affinity-
dc.subjectbinding assay-
dc.subjectBrucella abortus-
dc.subjectcell migration-
dc.subjectcell vacuole-
dc.subjectcholine uptake-
dc.subjectcontrolled study-
dc.subjectfluorescence-
dc.subjectgene disruption-
dc.subjectisotope labeling-
dc.subjectmutant-
dc.subjectnonhuman-
dc.subjectphospholipid synthesis-
dc.subjectpriority journal-
dc.subjectprotein analysis-
dc.subjectAmino Acid Sequence-
dc.subjectAnimals-
dc.subjectATP-Binding Cassette Transporters-
dc.subjectBacterial Proteins-
dc.subjectBiological Transport, Active-
dc.subjectBrucella abortus-
dc.subjectCell Membrane-
dc.subjectCholine-
dc.subjectFemale-
dc.subjectGene Expression Regulation, Bacterial-
dc.subjectMacrophages-
dc.subjectMice-
dc.subjectMice, Inbred BALB C-
dc.subjectMolecular Sequence Data-
dc.subjectOpen Reading Frames-
dc.subjectPhosphatidylcholines-
dc.titleIdentification and characterization of a high-affinity choline uptake system of Brucella abortus-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem