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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorOnken, H.-
dc.contributor<div class="autor_fcen" id="8656">Tresguerres, M.</div>-
dc.contributor<div class="autor_fcen" id="5211">Luquet, C.M.</div>-
dc.creatorOnken, H.-
dc.creator<div class="autor_fcen" id="8656">Tresguerres, M.</div>-
dc.creator<div class="autor_fcen" id="5211">Luquet, C.M.</div>-
dc.date.accessioned2018-05-04T22:01:13Z-
dc.date.accessioned2018-05-28T15:47:56Z-
dc.date.available2018-05-04T22:01:13Z-
dc.date.available2018-05-28T15:47:56Z-
dc.date.issued2003-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68385-
dc.descriptionSplit lamellae of posterior gills of Chasmagnathus granulatus adapted to 2.5‰ salinity were mounted in a modified Ussing chamber. With NaCl-saline on both sides of the preparation a transepithelial voltage (Vte) of 4.1±0.5 mV (outside positive) was measured. After voltage-clamping, the negative short-circuit current (Isc) amounted to -142±21 μA cm-2 at a conductance (Gte) of 44±5 mS cm-2. Substitution of either chloride (by nitrate) or sodium (by choline) on both sides of split gill lamellae significantly reduced Isc (by 70-80%) and Gte (by 30-50%). External CsCl (but not BaCl2 or furosemide) inhibited the negative Isc without affecting Gte. Addition of ouabain, BaCl2 or diphenylamine-2-carboxylate to the internal bath inhibited Isc at unchanged Gte. Internal acetazolamide did not affect Isc or Gte across split gill lamellae. Unidirectional Na+ influx across isolated and perfused posterior gills, however, was reduced by internal acetazolamide by approximately 20% at constant Vte. The results suggest that posterior gills of hyperosmoregulating C. granulatus display a high conductance epithelium that actively absorbs NaCl in a coupled way by an electrogenic mechanism similar to that seen in the thick ascending limb of Henle's loop and, to a minor degree, by an electroneutral mechanism, presumably via apical Na+/H+and Cl-/HCO3--antiports.-
dc.descriptionFil:Tresguerres, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourceJ. Exp. Biol. 2003;206(6):1017-1023-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_00220949_v206_n6_p1017_Onken.pdf-
dc.subjectChasmagnathus granulatus-
dc.subjectCrab gill-
dc.subjectCrustacea-
dc.subjectFlux measurement-
dc.subjectInhibitor-
dc.subjectIon substitution-
dc.subjectIon transport-
dc.subjectOsmoregulation-
dc.subjectShort-circuit current-
dc.subjectTransepithelial conductance-
dc.subjectUssing chamber-
dc.subjectacetazolamide-
dc.subjectanthranilic acid derivative-
dc.subjectfenamic acid-
dc.subjectouabain-
dc.subjectsodium chloride-
dc.subjectabsorption-
dc.subjectactive transport-
dc.subjectanimal-
dc.subjectarticle-
dc.subjectBrachyura-
dc.subjectcomparative study-
dc.subjectelectrolyte balance-
dc.subjectelectrophysiology-
dc.subjectgill-
dc.subjection transport-
dc.subjectmetabolism-
dc.subjectphysiology-
dc.subjectAbsorption-
dc.subjectAcetazolamide-
dc.subjectAnimals-
dc.subjectAnthranilic Acids-
dc.subjectBiological Transport, Active-
dc.subjectBrachyura-
dc.subjectElectrophysiology-
dc.subjectGills-
dc.subjectIon Transport-
dc.subjectOuabain-
dc.subjectSodium Chloride-
dc.subjectWater-Electrolyte Balance-
dc.subjectAnimalia-
dc.subjectBrachyura-
dc.subjectChasmagnathus granulata-
dc.subjectCrustacea-
dc.subjectDecapoda (Crustacea)-
dc.subjectStrophanthus gratus-
dc.titleActive NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

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