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  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorCroci, D.O.-
dc.contributorCumashi, A.-
dc.contributorUshakova, N.A.-
dc.contributorPreobrazhenskaya, M.E.-
dc.contributorPiccoli, A.-
dc.contributorTotani, L.-
dc.contributorUstyuzhanina, N.E.-
dc.contributorBilan, M.I.-
dc.contributorUsov, A.I.-
dc.contributorGrachev, A.A.-
dc.contributorMorozevich, G.E.-
dc.contributorBerman, A.E.-
dc.contributorSanderson, C.J.-
dc.contributorKelly, M.-
dc.contributorGregorio, P.-
dc.contributorRossi, C.-
dc.contributorTinari, N.-
dc.contributorIacobelli, S.-
dc.contributorRabinovich, G.A.-
dc.contributorNifantiev, N.E.-
dc.creatorCroci, D.O.-
dc.creatorCumashi, A.-
dc.creatorUshakova, N.A.-
dc.creatorPreobrazhenskaya, M.E.-
dc.creatorPiccoli, A.-
dc.creatorTotani, L.-
dc.creatorUstyuzhanina, N.E.-
dc.creatorBilan, M.I.-
dc.creatorUsov, A.I.-
dc.creatorGrachev, A.A.-
dc.creatorMorozevich, G.E.-
dc.creatorBerman, A.E.-
dc.creatorSanderson, C.J.-
dc.creatorKelly, M.-
dc.creatorGregorio, P.-
dc.creatorRossi, C.-
dc.creatorTinari, N.-
dc.creatorIacobelli, S.-
dc.creatorRabinovich, G.A.-
dc.creatorNifantiev, N.E.-
dc.date.accessioned2018-05-04T21:59:55Z-
dc.date.accessioned2018-05-28T15:49:03Z-
dc.date.available2018-05-04T21:59:55Z-
dc.date.available2018-05-28T15:49:03Z-
dc.date.issued2011-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68601-
dc.descriptionSulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characterize, using in vitro and in vivo strategies, the anti-inflammatory, anti-coagulant, anti-angiogenic, and anti-tumor activities of two main sulfated polysaccharide fractions obtained from L. saccharina: a) L.s.-1.0 fraction mainly consisting of O-sulfated mannoglucuronofucans and b) L.s.-1.25 fraction mainly composed of sulfated fucans. Both fractions inhibited leukocyte recruitment in a model of inflammation in rats, although L.s.-1.25 appeared to be more active than L.s.-1.0. Also, these fractions inhibited neutrophil adhesion to platelets under flow. Only fraction L.s.-1.25, but not L.s.-1.0, displayed anticoagulant activity as measured by the activated partial thromboplastin time. Investigation of these fractions in angiogenesis settings revealed that only L.s.-1.25 strongly inhibited fetal bovine serum (FBS) induced in vitro tubulogenesis. This effect correlated with a reduction in plasminogen activator inhibitor-1 (PAI-1) levels in L.s.-1.25-treated endothelial cells. Furthermore, only parent sulfated polysaccharides from L. saccharina (L.s.-P) and its fraction L.s.-1.25 were powerful inhibitors of basic fibroblast growth factor (bFGF) induced pathways. Consistently, the L.s.-1.25 fraction as well as L.s.-P successfully interfered with fibroblast binding to human bFGF. The incorporation of L.s.-P or L.s.-1.25, but not L.s.-1.0 into Matrigel plugs containing melanoma cells induced a significant reduction in hemoglobin content as well in the frequency of tumor-associated blood vessels. Moreover, i.p. administrations of L.s.-1.25, as well as L.s.-P, but not L.s.-1.0, resulted in a significant reduction of tumor growth when inoculated into syngeneic mice. Finally, L.s.-1.25 markedly inhibited breast cancer cell adhesion to human platelet-coated surfaces. Thus, sulfated fucans are mainly responsible for the anti-inflammatory, anticoagulant, antiangiogenic, and antitumor activities of sulfated polysaccharides from L. saccharina brown seaweed. © 2011 Croci et al.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourcePLoS ONE 2011;6(2)-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_19326203_v6_n2_p_Croci.pdf-
dc.subjectbovine serum albumin-
dc.subjectcarbohydrate derivative-
dc.subjectfibroblast growth factor-
dc.subjecthemoglobin-
dc.subjecto sulfated mannoglucuronofucan derivative-
dc.subjectplasminogen activator inhibitor 1-
dc.subjectpolysaccharide sulfate-
dc.subjectsulfated fucan derivative-
dc.subjectunclassified drug-
dc.subjectangiogenesis inhibitor-
dc.subjectanticoagulant agent-
dc.subjectantiinflammatory agent-
dc.subjectbiological product-
dc.subjectfucoidin-
dc.subjectfucose-
dc.subjectpolysaccharide-
dc.subjectangiogenesis-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectantiangiogenic activity-
dc.subjectanticoagulation-
dc.subjectantiinflammatory activity-
dc.subjectantineoplastic activity-
dc.subjectarticle-
dc.subjectbiological activity-
dc.subjectbreast cancer-
dc.subjectcancer growth-
dc.subjectcancer inhibition-
dc.subjectcell level-
dc.subjectcell surface-
dc.subjectcontrolled study-
dc.subjectdrug determination-
dc.subjectdrug efficacy-
dc.subjectdrug isolation-
dc.subjectdrug structure-
dc.subjectendothelium cell-
dc.subjectfibroblast-
dc.subjecthuman-
dc.subjecthuman cell-
dc.subjectin vitro study-
dc.subjectin vivo study-
dc.subjectLaminaria-
dc.subjectLaminaria saccharina-
dc.subjectleukocyte migration inhibition-
dc.subjectmelanoma-
dc.subjectmelanoma cell-
dc.subjectmouse-
dc.subjectnonhuman-
dc.subjectpartial thromboplastin time-
dc.subjectperitonitis-
dc.subjectrat-
dc.subjectthrombocyte adhesion-
dc.subjecttumor vascularization-
dc.subjectanimal-
dc.subjectbrown alga-
dc.subjectC57BL mouse-
dc.subjectcell culture-
dc.subjectchemistry-
dc.subjectdrug effect-
dc.subjectevaluation-
dc.subjectfemale-
dc.subjectinflammation-
dc.subjectmetabolism-
dc.subjectpathology-
dc.subjectphysiology-
dc.subjectseaweed-
dc.subjectWistar rat-
dc.subjectBovinae-
dc.subjectMus-
dc.subjectRattus-
dc.subjectSaccharina latissima-
dc.subjectAngiogenesis Inhibitors-
dc.subjectAnimals-
dc.subjectAnti-Inflammatory Agents-
dc.subjectAnticoagulants-
dc.subjectBiological Products-
dc.subjectCells, Cultured-
dc.subjectEndothelial Cells-
dc.subjectFemale-
dc.subjectFucose-
dc.subjectHumans-
dc.subjectInflammation-
dc.subjectLaminaria-
dc.subjectMice-
dc.subjectMice, Inbred C57BL-
dc.subjectNeovascularization, Physiologic-
dc.subjectPhaeophyta-
dc.subjectPolysaccharides-
dc.subjectRats-
dc.subjectRats, Wistar-
dc.subjectSeaweed-
dc.titleFucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

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