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  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorSala, C.D.-
dc.contributor<div class="autor_fcen" id="8203">Soler-Bistué, A.J.C.</div>-
dc.contributorKorprapun, L.-
dc.contributor<div class="autor_fcen" id="9341">Zorreguieta, A.</div>-
dc.contributor<div class="autor_fcen" id="8575">Tolmasky, M.E.</div>-
dc.creatorSala, C.D.-
dc.creator<div class="autor_fcen" id="8203">Soler-Bistué, A.J.C.</div>-
dc.creatorKorprapun, L.-
dc.creator<div class="autor_fcen" id="9341">Zorreguieta, A.</div>-
dc.creator<div class="autor_fcen" id="8575">Tolmasky, M.E.</div>-
dc.date.accessioned2018-05-04T22:02:55Z-
dc.date.accessioned2018-05-28T15:49:09Z-
dc.date.available2018-05-04T22:02:55Z-
dc.date.available2018-05-28T15:49:09Z-
dc.date.issued2012-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68613-
dc.descriptionEGS (external guide sequence) technology is a promising approach to designing new antibiotics. EGSs are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. The ftsZ mRNA secondary structure was modeled and EGSs complementary to two regions with high probability of being suitable targets were designed. In vitro reactions showed that EGSs targeting these regions bound ftsZ mRNA and elicited RNase P-mediated cleavage of ftsZ mRNA. A recombinant plasmid, pEGSb1, coding for an EGS that targets region "b" under the control of the T7 promoter was generated. Upon introduction of this plasmid into Escherichia coli BL21(DE3)(pLysS) the transformant strain formed filaments when expression of the EGS was induced. Concomitantly, E. coli harboring pEGSb1 showed a modest but significant inhibition of growth when synthesis of the EGSb1 was induced. Our results indicate that EGS technology could be a viable strategy to generate new antimicrobials targeting ftsZ. © 2012 Sala et al.-
dc.descriptionFil:Soler-Bistué, A.J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Tolmasky, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourcePLoS ONE 2012;7(10)-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_19326203_v7_n10_p_Sala.pdf-
dc.subjectmessenger RNA-
dc.subjectribonuclease P-
dc.subjecttransfer RNA-
dc.subjectarticle-
dc.subjectbacterial cell-
dc.subjectbinding affinity-
dc.subjectbinding site-
dc.subjectcomplex formation-
dc.subjectcontrolled study-
dc.subjectEscherichia coli-
dc.subjectexperimental design-
dc.subjectexternal guide sequence technology-
dc.subjectftsZ gene-
dc.subjectgene-
dc.subjectgene targeting-
dc.subjectgene technology-
dc.subjectgrowth inhibition-
dc.subjectin vitro study-
dc.subjectmitosis inhibition-
dc.subjectnonhuman-
dc.subjectpromoter region-
dc.subjectprotein secondary structure-
dc.subjectrecombinant plasmid-
dc.subjectRNA binding-
dc.subjectRNA cleavage-
dc.subjectRNA structure-
dc.subjectsequence analysis-
dc.subjectAnti-Bacterial Agents-
dc.subjectBacterial Proteins-
dc.subjectBase Sequence-
dc.subjectCell Division-
dc.subjectCytoskeletal Proteins-
dc.subjectDrug Design-
dc.subjectElectrophoretic Mobility Shift Assay-
dc.subjectEscherichia coli-
dc.subjectMicroscopy, Confocal-
dc.subjectMolecular Sequence Data-
dc.subjectNucleic Acid Conformation-
dc.subjectOligoribonucleotides, Antisense-
dc.subjectPromoter Regions, Genetic-
dc.subjectRibonuclease P-
dc.subjectRNA Cleavage-
dc.subjectTerminator Regions, Genetic-
dc.titleInhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
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