1. RODNA
  2. FCEN
  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributor<div class="autor_fcen" id="245">Álvarez, Y.D.</div>-
dc.contributor<div class="autor_fcen" id="744">Belingheri, A.V.</div>-
dc.contributor<div class="autor_fcen" id="6623">Perez Bay, A.E.</div>-
dc.contributorJavis, S.E.-
dc.contributorTedford, H.W.-
dc.contributorZamponi, G.-
dc.contributor<div class="autor_fcen" id="5377">Marengo, F.D.</div>-
dc.creator<div class="autor_fcen" id="245">Álvarez, Y.D.</div>-
dc.creator<div class="autor_fcen" id="744">Belingheri, A.V.</div>-
dc.creator<div class="autor_fcen" id="6623">Perez Bay, A.E.</div>-
dc.creatorJavis, S.E.-
dc.creatorTedford, H.W.-
dc.creatorZamponi, G.-
dc.creator<div class="autor_fcen" id="5377">Marengo, F.D.</div>-
dc.date.accessioned2018-05-04T21:58:37Z-
dc.date.accessioned2018-05-28T15:49:23Z-
dc.date.available2018-05-04T21:58:37Z-
dc.date.available2018-05-28T15:49:23Z-
dc.date.issued2013-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68640-
dc.descriptionIt is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al.-
dc.descriptionFil:Álvarez, Y.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Belingheri, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Perez Bay, A.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Marengo, F.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourcePLoS ONE 2013;8(1)-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_19326203_v8_n1_p_Alvarez.pdf-
dc.subjectcalcium channel P type-
dc.subjectcalcium channel Q type-
dc.subjectomega agatoxin IVA-
dc.subjectpotassium ion-
dc.subjectanimal cell-
dc.subjectarticle-
dc.subjectbinding site-
dc.subjectcalcium current-
dc.subjectcell interaction-
dc.subjectcell vacuole-
dc.subjectchromaffin cell-
dc.subjectcontrolled study-
dc.subjectdepolarization-
dc.subjectexocytosis-
dc.subjectmouse-
dc.subjectnerve cell stimulation-
dc.subjectnonhuman-
dc.subjectprotein expression-
dc.subjectprotein interaction-
dc.subjectsequence analysis-
dc.subjectsynapse-
dc.subjecttransient expression-
dc.subjectAnimals-
dc.subjectCalcium-
dc.subjectCalcium Channels, P-Type-
dc.subjectCalcium Channels, Q-Type-
dc.subjectCells, Cultured-
dc.subjectChromaffin Cells-
dc.subjectExocytosis-
dc.subjectMice-
dc.subjectSecretory Vesicles-
dc.titleThe Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem