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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Facultad de Ciencias Exactas y Naturales de la UBA | - |
| dc.contributor | Gomez, Daniel E. | - |
| dc.contributor | Tejera, Agueda Mercedes | - |
| dc.creator | Tejera, Agueda Mercedes | - |
| dc.date.accessioned | 2018-05-04T22:02:28Z | - |
| dc.date.accessioned | 2018-05-28T16:37:30Z | - |
| dc.date.available | 2018-05-04T22:02:28Z | - |
| dc.date.available | 2018-05-28T16:37:30Z | - |
| dc.date.issued | 2002 | - |
| dc.identifier.uri | http://10.0.0.11:8080/jspui/handle/bnmm/73893 | - |
| dc.description | Normal cells in culture divide a certain amount of times and undergo a process termed replicative senescence. Telomere loss is thought to control entry into senescence. Activation of telomerase in tumors bypasses cellular senescence and is thus a requirement for tumor progression. In this work, we have investigated the effects of chronic in vitro 3'-azido-2', 3'-dideoxythymidine (AZT)exposure on human and murine carcinoma cells. We demonstrate the irreversible telomere shortening in human cervical cancer cells (HeLa) cultured for long-term with AZT, but without evidence of senescence. Furthermore, we demonstrate, for the first time, that AZT-treated murine mammary carcinoma cells (F3II) have a reduced tumorigenicity in syngeneic BALB/c mice. Tumor incidence was reduced and survival was prolonged in animals inoculated with AZT-treated cells when comparing with control counterparts. The number and size of spontaneous metastases were also decreased in animals receiving AZT-treated F3II cells. In addition, we present morphological and biochemical evidence of senescence, and induction of apoptosis in tumor cells exposed to AZT. These data indicate that chronic exposure of mammary carcinoma cells to AZT may be sufficient to induce a senescent phenotype and to reduce tumorigenicity. | - |
| dc.description | Fil:Tejera, Agueda Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. | - |
| dc.format | application/pdf | - |
| dc.language | spa | - |
| dc.publisher | Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | http://creativecommons.org/licenses/by/2.5/ar | - |
| dc.source.uri | http://digital.bl.fcen.uba.ar/gsdl-282/cgi-bin/library.cgi?a=d&c=tesis&d=Tesis_3502_Tejera | - |
| dc.subject | APOPTOSIS | - |
| dc.subject | AZT | - |
| dc.subject | BREAST CANCER | - |
| dc.subject | SENESCENCE | - |
| dc.subject | TELOMERASE | - |
| dc.subject | APOPTOSIS | - |
| dc.subject | AZT | - |
| dc.subject | CANCER DE MAMA | - |
| dc.subject | SENESCENCIA | - |
| dc.subject | TELOMERASA | - |
| dc.title | Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad | - |
| dc.title | Effects of AZT prolonged treatment on tumor cells: telomere shortening, induction of senescence and apoptosis and reduction of tumorigenicity | - |
| dc.type | info:eu-repo/semantics/doctoralThesis | - |
| dc.type | info:ar-repo/semantics/tesis doctoral | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| Aparece en las colecciones: | FCEN - Facultad de Ciencias Exactas y Naturales. UBA | |
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